Detailed information of TA system TA032210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 46050..46681
• Replicon: chromosome
• Accession: NZ_AP018781
• Organism: Haemophilus influenzae strain CHBN-V-1 isolate 13H43

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CHBNTHi_RS00220
• Protein ID: WP_005648013.1
• Coordinates: 46283..46681 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q4QLV9
• Locus tag: CHBNTHi_RS00215
• Protein ID: WP_005648011.1
• Coordinates: 46050..46283 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHBNTHi_RS00190 (CHBNV1_00380)	41528..42730	-	1203	WP_005648002.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
CHBNTHi_RS00195 (CHBNV1_00390)	42894..43559	+	666	WP_020910156.1	DNA repair protein RadC	-
CHBNTHi_RS00200 (CHBNV1_00400)	43773..44009	+	237	WP_005542826.1	50S ribosomal protein L28	-
CHBNTHi_RS00205 (CHBNV1_00410)	44021..44191	+	171	WP_005613503.1	50S ribosomal protein L33	-
CHBNTHi_RS00210 (CHBNV1_00420)	44539..45903	+	1365	WP_005648009.1	diaminobutyrate--2-oxoglutarate transaminase	-
CHBNTHi_RS00215 (CHBNV1_00430)	46050..46283	+	234	WP_005648011.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
CHBNTHi_RS00220 (CHBNV1_00440)	46283..46681	+	399	WP_005648013.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CHBNTHi_RS00225 (CHBNV1_00450)	46701..48236	+	1536	WP_005648022.1	L-2,4-diaminobutyrate decarboxylase	-
CHBNTHi_RS00230 (CHBNV1_00460)	48468..49283	+	816	WP_005648023.1	DNA-formamidopyrimidine glycosylase	-
CHBNTHi_RS00235 (CHBNV1_00470)	49355..50377	-	1023	WP_005648024.1	outer membrane-stress sensor serine endopeptidase DegS	-
CHBNTHi_RS00240 (CHBNV1_00480)	50378..51496	-	1119	WP_075985380.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15073.38 Da
• Isoelectric Point: 8.0514

>T32210 WP_005648013.1 NZ_AP018781:46283-46681 [Haemophilus influenzae]
VLKYMLDTNIVIYVIKRRPLEILSRFNQNAGKMCVSSITVAELYYGAEKSEYPERNIAGIEDFLSRLTILDYQPKHAAHF
GNIKAELSKQGKLIGENDIHIAAHARSEGLILVSNNLREFERVIALRTENWV

Nucleotide

• Length: 399 bp

>T32210 NZ_AP018781:46283-46681 [Haemophilus influenzae]
GTGTTGAAATATATGCTTGATACTAACATTGTGATTTACGTGATTAAGCGTAGGCCATTGGAAATTCTTTCTCGCTTTAA
TCAAAATGCAGGAAAAATGTGCGTGAGTAGCATTACTGTGGCTGAACTCTATTATGGAGCTGAAAAAAGCGAATATCCCG
AAAGAAACATTGCGGGTATTGAAGATTTCTTATCACGCCTCACCATTTTAGATTACCAACCAAAGCATGCAGCTCATTTC
GGCAATATTAAAGCTGAACTCTCTAAACAAGGGAAATTGATTGGCGAAAATGATATTCATATAGCGGCTCATGCTCGTAG
TGAAGGGTTAATTCTGGTTAGTAACAATTTAAGAGAATTTGAGCGAGTTATAGCATTACGTACAGAAAATTGGGTGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8880.98 Da
• Isoelectric Point: 4.4013

>AT32210 WP_005648011.1 NZ_AP018781:46050-46283 [Haemophilus influenzae]
MIEASVFMTNRSQAVRLPAEVRFSEEIKKLSVRVSGSDRILSPLNQSWDSFFLNDQAVSDDFMNEREIAFQPEREAL

Nucleotide

• Length: 234 bp

>AT32210 NZ_AP018781:46050-46283 [Haemophilus influenzae]
ATGATTGAAGCCTCTGTTTTTATGACTAATCGAAGTCAAGCTGTGCGTTTGCCCGCTGAAGTTCGCTTTTCTGAAGAAAT
CAAAAAATTATCGGTTCGTGTATCGGGCAGTGATCGGATTTTATCGCCTCTCAACCAATCTTGGGATAGTTTTTTCTTGA
ATGATCAAGCAGTTAGCGATGATTTTATGAATGAACGAGAAATTGCATTTCAACCAGAGCGTGAGGCTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A806DGS0