Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) VapXD/-
Location 576991..577469 Replicon chromosome
Accession NZ_AP018780
Organism Haemophilus influenzae strain CHBN-IV-1 isolate 13H36

Toxin (Protein)


Gene name VapD Uniprot ID A0A0H3PEZ5
Locus tag K6J73_RS02870 Protein ID WP_005656500.1
Coordinates 577191..577469 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name VapX Uniprot ID A0A0M1GIA6
Locus tag K6J73_RS02865 Protein ID WP_005649350.1
Coordinates 576991..577182 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J73_RS02840 (CHBNIV1_05340) 572570..573202 + 633 WP_005662579.1 dTMP kinase -
K6J73_RS02845 (CHBNIV1_05350) 573199..574182 + 984 WP_005662580.1 DNA polymerase III subunit delta' -
K6J73_RS02850 (CHBNIV1_05360) 574237..575019 + 783 WP_005662581.1 YchF/TatD family DNA exonuclease -
K6J73_RS02855 (CHBNIV1_05370) 575046..575570 + 525 WP_005656502.1 DUF1523 family protein -
K6J73_RS02860 (CHBNIV1_05380) 575621..576919 + 1299 WP_075985482.1 hemolysin family protein -
K6J73_RS02865 (CHBNIV1_05390) 576991..577182 + 192 WP_005649350.1 DUF5397 domain-containing protein Antitoxin
K6J73_RS02870 (CHBNIV1_05400) 577191..577469 + 279 WP_005656500.1 virulence-associated protein VapD Toxin
K6J73_RS02875 (CHBNIV1_05410) 577562..578092 + 531 WP_221253376.1 hypothetical protein -
K6J73_RS02880 (CHBNIV1_05420) 578164..579663 + 1500 WP_005669277.1 fused PTS fructose transporter subunit IIA/HPr protein -
K6J73_RS02885 (CHBNIV1_05430) 579665..580606 + 942 WP_221253380.1 1-phosphofructokinase -
K6J73_RS02890 (CHBNIV1_05440) 580608..582278 + 1671 WP_005669262.1 fructose-specific PTS transporter subunit EIIC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Virulence-associated protein D / CRISPR associated protein Cas2

(1-86)

Antitoxin

Protein of unknown function DUF5397

(1-63)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.09 Da        Isoelectric Point: 5.7408

>T32207 WP_005656500.1 NZ_AP018780:577191-577469 [Haemophilus influenzae]
MYAIAFDLVVKDTQDYHPKGVQQAYTDIGAVLAKFGFVRTQGSLYTNMNEDMANLFQAMNALKQLAWISQSVRDIRAFRI
EQWSDFTDFIRN

Download         Length: 279 bp

>T32207 NZ_AP018780:577191-577469 [Haemophilus influenzae]
ATGTACGCGATTGCTTTTGATTTAGTTGTAAAAGACACTCAAGATTATCATCCAAAGGGTGTTCAACAGGCTTATACAGA
CATTGGCGCAGTTTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATACCAATATGAATGAAGATATGGCGA
ATCTCTTTCAGGCAATGAATGCACTAAAACAGTTGGCGTGGATTTCTCAGTCTGTACGAGATATTCGCGCTTTTCGTATT
GAGCAATGGTCTGATTTTACTGATTTTATACGAAATTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7047.02 Da        Isoelectric Point: 3.9930

>AT32207 WP_005649350.1 NZ_AP018780:576991-577182 [Haemophilus influenzae]
MELRQQIPTGCIKQFGQFGVPYVVGEVAEFLPDGDVLVNITLLQSGEKDIYRLSHLLEDPEAE

Download         Length: 192 bp

>AT32207 NZ_AP018780:576991-577182 [Haemophilus influenzae]
ATGGAACTAAGACAACAAATTCCAACAGGCTGTATTAAGCAATTTGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGTGATGTGTTGGTGAATATCACATTGTTACAATCTGGTGAAAAAGACATTTATCGCTTAT
CTCACCTGCTTGAAGATCCCGAAGCTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3PEZ5

Antitoxin

Source ID Structure
AlphaFold DB A0A0M1GIA6

References