Detailed information of TA system TA032200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 582610..583088
• Replicon: chromosome
• Accession: NZ_AP018779
• Organism: Haemophilus influenzae strain CHBN-III-8 isolate 10H15

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: -
• Locus tag: K6J62_RS02910
• Protein ID: WP_110431606.1
• Coordinates: 582810..583088 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: A0A0K9KX05
• Locus tag: K6J62_RS02905
• Protein ID: WP_012055040.1
• Coordinates: 582610..582801 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J62_RS02885 (CHBNIII8_05450)	578837..579817	+	981	WP_110431610.1	DNA polymerase III subunit delta'	-
K6J62_RS02890 (CHBNIII8_05460)	579856..580638	+	783	WP_110431609.1	YchF/TatD family DNA exonuclease	-
K6J62_RS02895 (CHBNIII8_05470)	580665..581189	+	525	WP_110431608.1	DUF1523 family protein	-
K6J62_RS02900 (CHBNIII8_05480)	581240..582538	+	1299	WP_110431607.1	hemolysin family protein	-
K6J62_RS02905 (CHBNIII8_05490)	582610..582801	+	192	WP_012055040.1	DUF5397 family protein	Antitoxin
K6J62_RS02910 (CHBNIII8_05500)	582810..583088	+	279	WP_110431606.1	virulence-associated protein VapD	Toxin
K6J62_RS02915 (CHBNIII8_05510)	583184..583714	+	531	WP_005662583.1	hypothetical protein	-
K6J62_RS02920 (CHBNIII8_05520)	583786..585285	+	1500	WP_110431605.1	fused PTS fructose transporter subunit IIA/HPr protein	-
K6J62_RS02925 (CHBNIII8_05530)	585287..586228	+	942	WP_110431604.1	1-phosphofructokinase	-
K6J62_RS02930 (CHBNIII8_05540)	586230..587900	+	1671	WP_110431603.1	fructose-specific PTS transporter subunit EIIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (1-63)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10736.13 Da
• Isoelectric Point: 4.9670

>T32200 WP_110431606.1 NZ_AP018779:582810-583088 [Haemophilus influenzae]
MYAIAFDLVVKDTQDYHPKGVQQAYTDIGETLAKFGFVRTQGSLYTNMNEDMANLFQAMNALKQLAWISQSVRDIRTFRI
EQWSDFTDFIRN

Nucleotide

• Length: 279 bp

>T32200 NZ_AP018779:582810-583088 [Haemophilus influenzae]
ATGTATGCGATTGCTTTTGATTTAGTTGTAAAAGACACTCAAGATTATCATCCAAAGGGCGTTCAACAGGCTTATACAGA
CATTGGCGAAACCTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATACCAATATGAATGAAGATATGGCGA
ATCTTTTTCAGGCAATGAATGCACTAAAACAGTTGGCGTGGATTTCTCAGTCGGTACGAGATATTCGCACTTTTCGTATT
GAGCAATGGTCTGATTTTACTGATTTTATACGAAATTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7018.97 Da
• Isoelectric Point: 3.9930

>AT32200 WP_012055040.1 NZ_AP018779:582610-582801 [Haemophilus influenzae]
MELRQQIPTGCIKQFGQFGVPYVVGEVAEFLPDGDALVNITLLQSGEKDIYRLSHLLEDPEAE

Nucleotide

• Length: 192 bp

>AT32200 NZ_AP018779:582610-582801 [Haemophilus influenzae]
ATGGAACTAAGACAACAAATTCCAACAGGCTGTATTAAGCAATTCGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGCGATGCGTTGGTGAATATCACATTATTACAATCTGGAGAAAAAGACATTTATCGCTTAT
CTCACCTACTTGAAGATCCCGAAGCTGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0K9KX05