Detailed information of TA system TA032199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 338705..339215
• Replicon: chromosome
• Accession: NZ_AP018779
• Organism: Haemophilus influenzae strain CHBN-III-8 isolate 10H15

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A2S9R6L1
• Locus tag: K6J62_RS01685
• Protein ID: WP_038439526.1
• Coordinates: 338705..338926 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: K6J62_RS01690
• Protein ID: WP_005650837.1
• Coordinates: 338919..339215 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J62_RS08765	335653..335703	+	51	Protein_326	hypothetical protein	-
K6J62_RS08780	335751..336135	+	385	Protein_327	TonB-dependent receptor plug domain-containing protein	-
K6J62_RS01680 (CHBNIII8_03220)	336343..338598	+	2256	WP_246588190.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
K6J62_RS01685 (CHBNIII8_03230)	338705..338926	+	222	WP_038439526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J62_RS01690 (CHBNIII8_03240)	338919..339215	+	297	WP_005650837.1	helix-turn-helix transcriptional regulator	Antitoxin
K6J62_RS01695 (CHBNIII8_03250)	339382..341298	+	1917	WP_110431987.1	ABC transporter ATP-binding protein	-
K6J62_RS01700 (CHBNIII8_03260)	341308..341844	+	537	WP_110431986.1	type I DNA topoisomerase	-
K6J62_RS01705 (CHBNIII8_03270)	341860..342411	+	552	WP_110431985.1	Sua5/YciO/YrdC/YwlC family protein	-
K6J62_RS01710 (CHBNIII8_03280)	342415..343233	+	819	WP_110431984.1	shikimate dehydrogenase	-
K6J62_RS01715 (CHBNIII8_03290)	343230..343787	+	558	WP_110431983.1	DNA-3-methyladenine glycosylase I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-63)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8804.33 Da
• Isoelectric Point: 10.7588

>T32199 WP_038439526.1 NZ_AP018779:338705-338926 [Haemophilus influenzae]
VQNLLENQALFAILDGEIWELRPIRDRILFARLMDGRFVLLHQFMKKTQKTPKREIQTAQQRLSELKERLKNE

Nucleotide

• Length: 222 bp

>T32199 NZ_AP018779:338705-338926 [Haemophilus influenzae]
GTGCAAAATCTCCTTGAAAATCAAGCGCTTTTTGCTATTTTAGACGGTGAAATTTGGGAACTTCGCCCAATTAGGGATCG
CATCTTGTTTGCAAGATTAATGGACGGTCGCTTTGTCTTACTACATCAATTTATGAAGAAAACACAAAAAACGCCAAAAC
GAGAAATCCAAACTGCCCAACAACGCTTAAGTGAATTAAAAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11092.94 Da
• Isoelectric Point: 10.3139

>AT32199 WP_005650837.1 NZ_AP018779:338919-339215 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide

• Length: 297 bp

>AT32199 NZ_AP018779:338919-339215 [Haemophilus influenzae]
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAGCAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAACTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGGGTAAAAAATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A2S9R6L1