Detailed information of TA system TA032190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 46414..47045
• Replicon: chromosome
• Accession: NZ_AP018778
• Organism: Haemophilus influenzae strain CHBN-III-7 isolate 15H01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K6J60_RS00225
• Protein ID: WP_118807356.1
• Coordinates: 46647..47045 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q4QLV9
• Locus tag: K6J60_RS00220
• Protein ID: WP_005648011.1
• Coordinates: 46414..46647 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J60_RS00195 (CHBNIII7_00390)	41892..43094	-	1203	WP_221267335.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
K6J60_RS00200 (CHBNIII7_00400)	43258..43923	+	666	WP_118807354.1	DNA repair protein RadC	-
K6J60_RS00205 (CHBNIII7_00410)	44137..44373	+	237	WP_005542826.1	50S ribosomal protein L28	-
K6J60_RS00210 (CHBNIII7_00420)	44385..44555	+	171	WP_005613503.1	50S ribosomal protein L33	-
K6J60_RS00215 (CHBNIII7_00430)	44903..46267	+	1365	WP_118807355.1	diaminobutyrate--2-oxoglutarate transaminase	-
K6J60_RS00220 (CHBNIII7_00440)	46414..46647	+	234	WP_005648011.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
K6J60_RS00225 (CHBNIII7_00450)	46647..47045	+	399	WP_118807356.1	tRNA(fMet)-specific endonuclease VapC	Toxin
K6J60_RS00230 (CHBNIII7_00460)	47065..48600	+	1536	WP_118807357.1	L-2,4-diaminobutyrate decarboxylase	-
K6J60_RS00235 (CHBNIII7_00470)	48832..49647	+	816	WP_118807358.1	DNA-formamidopyrimidine glycosylase	-
K6J60_RS00240 (CHBNIII7_00480)	49721..50743	-	1023	WP_118807359.1	outer membrane-stress sensor serine endopeptidase DegS	-
K6J60_RS00245 (CHBNIII7_00490)	50744..51862	-	1119	WP_118807360.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15114.52 Da
• Isoelectric Point: 9.3215

>T32190 WP_118807356.1 NZ_AP018778:46647-47045 [Haemophilus influenzae]
VLKYMLDTNIVIYVIKRRPLEILSRFNQNAGKMCVSSITVAELYYGAEKSKYPERNIAVIEDFLSRLTILDYQPKHAAHF
GNIKAELSKQGKLIGENDIHIAAHARSEGLILVSNNLREFERVIALRTENWV

Nucleotide

• Length: 399 bp

>T32190 NZ_AP018778:46647-47045 [Haemophilus influenzae]
GTGTTGAAATATATGCTTGATACTAACATTGTGATTTACGTGATTAAGCGTAGGCCATTGGAAATTCTTTCTCGCTTTAA
TCAAAATGCAGGAAAAATGTGCGTGAGTAGCATTACTGTGGCTGAACTCTATTATGGAGCTGAAAAAAGCAAATATCCCG
AAAGAAACATTGCGGTTATTGAAGATTTCTTATCACGCCTCACCATTTTAGATTACCAACCAAAGCATGCAGCTCATTTC
GGCAATATTAAAGCTGAACTCTCTAAACAAGGGAAATTGATTGGCGAAAATGATATTCATATAGCTGCTCATGCTCGTAG
TGAAGGGTTAATTCTGGTTAGTAACAATTTAAGAGAATTTGAGCGAGTTATAGCATTACGTACAGAAAATTGGGTGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8880.98 Da
• Isoelectric Point: 4.4013

>AT32190 WP_005648011.1 NZ_AP018778:46414-46647 [Haemophilus influenzae]
MIEASVFMTNRSQAVRLPAEVRFSEEIKKLSVRVSGSDRILSPLNQSWDSFFLNDQAVSDDFMNEREIAFQPEREAL

Nucleotide

• Length: 234 bp

>AT32190 NZ_AP018778:46414-46647 [Haemophilus influenzae]
ATGATTGAAGCCTCTGTTTTTATGACTAATCGAAGTCAAGCTGTGCGTTTGCCCGCTGAAGTTCGCTTTTCTGAAGAAAT
CAAAAAATTATCGGTTCGTGTATCGGGCAGTGATCGGATTTTATCGCCTCTCAACCAATCTTGGGATAGTTTTTTCTTGA
ATGATCAAGCAGTTAGCGATGATTTTATGAATGAACGAGAAATTGCATTTCAACCAGAGCGTGAGGCTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A806DGS0