32184

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) VapXD/-
Location 187298..187776 Replicon chromosome
Accession NZ_AP018777
Organism Haemophilus influenzae strain CHBN-III-6 isolate 13H31

Toxin (Protein)


Gene name VapD Uniprot ID A0A0K9L551
Locus tag K6J69_RS00995 Protein ID WP_050848517.1
Coordinates 187298..187576 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name VapX Uniprot ID A0A0K9KX05
Locus tag K6J69_RS01000 Protein ID WP_012055040.1
Coordinates 187585..187776 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J69_RS00975 (CHBNIII6_01930) 182488..184158 - 1671 WP_110430653.1 fructose-specific PTS transporter subunit EIIC -
K6J69_RS00980 (CHBNIII6_01940) 184160..185101 - 942 WP_110430654.1 1-phosphofructokinase -
K6J69_RS00985 (CHBNIII6_01950) 185103..186602 - 1500 WP_110430655.1 fused PTS fructose transporter subunit IIA/HPr protein -
K6J69_RS00990 (CHBNIII6_01960) 186672..187202 - 531 WP_192876582.1 hypothetical protein -
K6J69_RS00995 (CHBNIII6_01970) 187298..187576 - 279 WP_050848517.1 virulence-associated protein VapD Toxin
K6J69_RS01000 (CHBNIII6_01980) 187585..187776 - 192 WP_012055040.1 DUF5397 family protein Antitoxin
K6J69_RS01005 (CHBNIII6_01990) 187847..189145 - 1299 WP_050846548.1 hemolysin family protein -
K6J69_RS01010 (CHBNIII6_02000) 189196..189720 - 525 WP_005669286.1 DUF1523 family protein -
K6J69_RS01015 (CHBNIII6_02010) 189747..190529 - 783 WP_110430657.1 YchF/TatD family DNA exonuclease -
K6J69_RS01020 (CHBNIII6_02020) 190568..191548 - 981 WP_110430691.1 DNA polymerase III subunit delta' -
K6J69_RS01025 (CHBNIII6_02030) 191545..192177 - 633 WP_192876583.1 dTMP kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Virulence-associated protein D / CRISPR associated protein Cas2

(1-86)

Antitoxin

Protein of unknown function DUF5397

(1-63)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10604.98 Da        Isoelectric Point: 5.7408

>T32184 WP_050848517.1 NZ_AP018777:c187576-187298 [Haemophilus influenzae]
MYAIAFDLVVKDTQDYHPKGVQQAYTDIGAVLAKFGFVRTQGSLYTNTNEDMANLFQAMNSLKQLAWISQSVRDIRAFRI
EQWSDFTDFIRS

Download         Length: 279 bp

>T32184 NZ_AP018777:c187576-187298 [Haemophilus influenzae]
ATGTACGCGATTGCTTTTGATTTAGTTGTAAAAGACACTCAAGATTATCATCCAAAAGGTGTTCAGCAGGCTTATACAGA
CATTGGTGCGGTTTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATACCAATACAAATGAAGATATGGCAA
ACCTTTTTCAGGCAATGAATTCGCTAAAACAGTTGGCGTGGATTTCTCAGTCGGTACGAGATATTCGTGCTTTTCGTATT
GAGCAATGGTCTGACTTCACTGACTTTATACGAAGTTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7018.97 Da        Isoelectric Point: 3.9930

>AT32184 WP_012055040.1 NZ_AP018777:c187776-187585 [Haemophilus influenzae]
MELRQQIPTGCIKQFGQFGVPYVVGEVAEFLPDGDALVNITLLQSGEKDIYRLSHLLEDPEAE

Download         Length: 192 bp

>AT32184 NZ_AP018777:c187776-187585 [Haemophilus influenzae]
ATGGAACTAAGACAACAAATTCCAACGGGCTGTATTAAACAATTCGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGCGATGCGTTGGTAAATATCACATTGTTACAATCTGGAGAAAAAGATATTTATCGCTTAT
CTCACTTACTTGAAGATCCCGAAGCTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K9L551

Antitoxin

Source ID Structure
AlphaFold DB A0A0K9KX05

References