Detailed information of TA system TA032180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 366745..367216
• Replicon: chromosome
• Accession: NZ_AP018776
• Organism: Haemophilus influenzae strain CHBN-III-5 isolate 13H30

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A806DTA9
• Locus tag: K6J67_RS01815
• Protein ID: WP_005654546.1
• Coordinates: 366745..366927 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: K6J67_RS01820
• Protein ID: WP_005650837.1
• Coordinates: 366920..367216 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J67_RS08945	363490..363633	+	144	WP_246588187.1	PT domain-containing protein	-
K6J67_RS08950	363575..363985	+	411	Protein_353	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
K6J67_RS01810 (CHBNIII5_03500)	364193..366475	+	2283	WP_246588188.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
K6J67_RS01815 (CHBNIII5_03510)	366745..366927	+	183	WP_005654546.1	shikimate 5-dehydrogenase	Toxin
K6J67_RS01820 (CHBNIII5_03520)	366920..367216	+	297	WP_005650837.1	helix-turn-helix transcriptional regulator	Antitoxin
K6J67_RS01825 (CHBNIII5_03530)	367400..369316	+	1917	WP_050822748.1	ABC transporter ATP-binding protein	-
K6J67_RS01830 (CHBNIII5_03540)	369326..369874	+	549	WP_050822746.1	type I DNA topoisomerase	-
K6J67_RS01835 (CHBNIII5_03550)	369890..370441	+	552	WP_050822744.1	Sua5/YciO/YrdC/YwlC family protein	-
K6J67_RS01840 (CHBNIII5_03560)	370445..371251	+	807	WP_050822741.1	shikimate dehydrogenase	-
K6J67_RS01845 (CHBNIII5_03570)	371465..372028	+	564	WP_042601571.1	DNA-3-methyladenine glycosylase I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7318.33 Da
• Isoelectric Point: 6.3057

>T32180 WP_005654546.1 NZ_AP018776:366745-366927 [Haemophilus influenzae]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVIQTAQQRLSELKERLKNE

Nucleotide

• Length: 183 bp

>T32180 NZ_AP018776:366745-366927 [Haemophilus influenzae]
ATGTACGAGATTCTCTTTTACAGAGACCAAAATGACATTGAGCCAGTGAAAGAGTATCTGCTATCGCTGGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAATCCAAACAGCCCAACAACGCTTAAGTGAATTAA
AAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11092.94 Da
• Isoelectric Point: 10.3139

>AT32180 WP_005650837.1 NZ_AP018776:366920-367216 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide

• Length: 297 bp

>AT32180 NZ_AP018776:366920-367216 [Haemophilus influenzae]
ATGAATAAAATTAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGTCAGCAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAACTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGGGTAAAAAATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A806DTA9