Detailed information of TA system TA032171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 365033..365504
• Replicon: chromosome
• Accession: NZ_AP018775
• Organism: Haemophilus influenzae strain CHBN-III-4 isolate 12H35

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A806DTA9
• Locus tag: K6J64_RS01835
• Protein ID: WP_005654546.1
• Coordinates: 365033..365215 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: K6J64_RS01840
• Protein ID: WP_005650837.1
• Coordinates: 365208..365504 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J64_RS08975	361778..361933	+	156	WP_233993456.1	PT domain-containing protein	-
K6J64_RS08980	361869..362288	+	420	Protein_362	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
K6J64_RS01830 (CHBNIII4_03540)	362496..364763	+	2268	WP_230304801.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
K6J64_RS01835 (CHBNIII4_03550)	365033..365215	+	183	WP_005654546.1	shikimate 5-dehydrogenase	Toxin
K6J64_RS01840 (CHBNIII4_03560)	365208..365504	+	297	WP_005650837.1	helix-turn-helix transcriptional regulator	Antitoxin
K6J64_RS01845 (CHBNIII4_03570)	365688..367604	+	1917	WP_048946754.1	ABC transporter ATP-binding protein	-
K6J64_RS01850 (CHBNIII4_03580)	367614..368150	+	537	WP_042594704.1	type I DNA topoisomerase	-
K6J64_RS01855 (CHBNIII4_03590)	368166..368717	+	552	WP_048946753.1	Sua5/YciO/YrdC/YwlC family protein	-
K6J64_RS01860 (CHBNIII4_03600)	368721..369539	+	819	WP_048946752.1	shikimate dehydrogenase	-
K6J64_RS01865 (CHBNIII4_03610)	369536..370093	+	558	WP_048946751.1	DNA-3-methyladenine glycosylase I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7318.33 Da
• Isoelectric Point: 6.3057

>T32171 WP_005654546.1 NZ_AP018775:365033-365215 [Haemophilus influenzae]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVIQTAQQRLSELKERLKNE

Nucleotide

• Length: 183 bp

>T32171 NZ_AP018775:365033-365215 [Haemophilus influenzae]
ATGTACGAGATTCTCTTTTACAGAGACCAAAATGACATTGAGCCAGTGAAAGAGTATCTGCTATCTCTAGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAATCCAAACAGCCCAACAACGCTTAAGTGAATTAA
AAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11092.94 Da
• Isoelectric Point: 10.3139

>AT32171 WP_005650837.1 NZ_AP018775:365208-365504 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide

• Length: 297 bp

>AT32171 NZ_AP018775:365208-365504 [Haemophilus influenzae]
ATGAATAAAATTAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAGCAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAGCTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGAGTAAAAAATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A806DTA9