Detailed information of TA system TA032169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 304057..304661
• Replicon: chromosome
• Accession: NZ_AP018775
• Organism: Haemophilus influenzae strain CHBN-III-4 isolate 12H35

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K6J64_RS01545
• Protein ID: WP_014551096.1
• Coordinates: 304057..304365 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q4QMK8
• Locus tag: K6J64_RS01550
• Protein ID: WP_005692346.1
• Coordinates: 304365..304661 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J64_RS01535 (CHBNIII4_02960)	299128..300426	-	1299	WP_014551094.1	trigger factor	-
K6J64_RS08945	300755..300853	+	99	Protein_301	PT domain-containing protein	-
K6J64_RS08955	300877..300960	+	84	Protein_302	PT domain-containing protein	-
K6J64_RS08960	300931..301312	+	382	Protein_303	TonB-dependent receptor plug domain-containing protein	-
K6J64_RS01540 (CHBNIII4_02970)	301739..304000	+	2262	WP_246588185.1	TonB-dependent receptor	-
K6J64_RS01545 (CHBNIII4_02980)	304057..304365	-	309	WP_014551096.1	type II toxin-antitoxin system YafQ family toxin	Toxin
K6J64_RS01550 (CHBNIII4_02990)	304365..304661	-	297	WP_005692346.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
K6J64_RS01555 (CHBNIII4_03000)	304781..304906	-	126	WP_041174648.1	selenocysteine synthase	-
K6J64_RS01560 (CHBNIII4_03010)	304890..306749	-	1860	WP_014551097.1	selenocysteine-specific translation elongation factor	-
K6J64_RS01565 (CHBNIII4_03020)	306746..308131	-	1386	WP_014551098.1	L-seryl-tRNA(Sec) selenium transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (7-100)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-93)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11903.65 Da
• Isoelectric Point: 7.3180

>T32169 WP_014551096.1 NZ_AP018775:c304365-304057 [Haemophilus influenzae]
MSEEKPLKVSYSKQFVRDLTDLAKRSPNVLIGSKYITAIYCLLNRLPLPESYQDHALVGEWKGYRDCHIQGDLVLIYQYV
IHDEFDELKFSRLNTHSQTALK

Nucleotide

• Length: 309 bp

>T32169 NZ_AP018775:c304365-304057 [Haemophilus influenzae]
ATGTCAGAAGAGAAGCCATTAAAAGTAAGCTATTCAAAACAATTTGTAAGAGATTTAACTGATTTAGCTAAACGCTCCCC
CAATGTGTTAATTGGCTCAAAATACATTACTGCAATATACTGTTTATTAAATCGTTTACCTTTACCCGAAAGCTACCAAG
ATCACGCATTAGTAGGAGAGTGGAAAGGTTATCGAGATTGCCATATTCAAGGCGATTTGGTACTGATTTACCAATACGTT
ATACACGATGAATTTGATGAATTGAAATTTTCTCGTTTAAATACACACTCACAAACCGCTTTAAAATAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11003.27 Da
• Isoelectric Point: 4.2845

>AT32169 WP_005692346.1 NZ_AP018775:c304661-304365 [Haemophilus influenzae]
MALTNSSISFRTVEQTKSEAYQVIEQYGLTPSQVFNMFLAQIAKTRSIPIDLNYLRPNKETLAAIDELDSGNAESFFIEA
SENYSAEEFTKRILNGGQ

Nucleotide

• Length: 297 bp

>AT32169 NZ_AP018775:c304661-304365 [Haemophilus influenzae]
ATGGCACTTACGAATTCCTCTATCAGCTTTAGAACAGTTGAGCAAACAAAATCAGAAGCTTATCAAGTTATTGAACAATA
TGGACTTACCCCATCACAAGTATTCAATATGTTTTTGGCTCAAATTGCCAAGACTCGCTCTATCCCTATTGATTTGAATT
ATCTTCGCCCAAATAAAGAAACCCTAGCAGCTATTGATGAATTAGATAGTGGTAACGCAGAGAGCTTTTTTATTGAAGCT
AGTGAAAATTATTCTGCCGAAGAATTTACAAAACGCATTCTTAATGGTGGTCAATAA

Structures

Antitoxin

• AlphaFold DB: Q4QMK8