Detailed information of TA system TA032168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 75454..75988
• Replicon: chromosome
• Accession: NZ_AP018775
• Organism: Haemophilus influenzae strain CHBN-III-4 isolate 12H35

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K6J64_RS00375
• Protein ID: WP_014551013.1
• Coordinates: 75698..75988 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E7A5Y2
• Locus tag: K6J64_RS00370
• Protein ID: WP_005651514.1
• Coordinates: 75454..75708 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J64_RS00335 (CHBNIII4_00680)	70634..70894	+	261	WP_005663579.1	hypothetical protein	-
K6J64_RS00340 (CHBNIII4_00690)	70924..71289	+	366	WP_005686191.1	endonuclease domain-containing protein	-
K6J64_RS00345 (CHBNIII4_00700)	71627..73693	+	2067	WP_005686190.1	glycine--tRNA ligase subunit beta	-
K6J64_RS00355 (CHBNIII4_00710)	74055..74594	+	540	WP_005659144.1	hypothetical protein	-
K6J64_RS00360 (CHBNIII4_00720)	74624..74932	+	309	WP_005659142.1	P2 family phage major capsid protein	-
K6J64_RS00365 (CHBNIII4_00730)	74932..75156	+	225	WP_005659141.1	hypothetical protein	-
K6J64_RS00370 (CHBNIII4_00740)	75454..75708	+	255	WP_005651514.1	stability protein StbD	Antitoxin
K6J64_RS00375 (CHBNIII4_00750)	75698..75988	+	291	WP_014551013.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J64_RS00380 (CHBNIII4_00760)	76024..76278	+	255	WP_014551014.1	hypothetical protein	-
K6J64_RS00385 (CHBNIII4_00780)	76448..76585	+	138	WP_005659136.1	hypothetical protein	-
K6J64_RS00390 (CHBNIII4_00790)	76808..77068	-	261	WP_221260091.1	hypothetical protein	-
K6J64_RS00395 (CHBNIII4_00800)	77260..78294	-	1035	WP_005659128.1	DNA polymerase III subunit delta	-
K6J64_RS00400 (CHBNIII4_00810)	78294..78797	-	504	WP_005659127.1	LPS assembly lipoprotein LptE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11498.41 Da
• Isoelectric Point: 10.6484

>T32168 WP_014551013.1 NZ_AP018775:75698-75988 [Haemophilus influenzae]
MTYKLIFDKRALKEWNKLGETLRQQFKNKLAERMINPRIQADKLKGENDLYKIKLRSAGYRLVYQVRDQEITIIVVSVGK
RERLEVYKTAEQRTSH

Nucleotide

• Length: 291 bp

>T32168 NZ_AP018775:75698-75988 [Haemophilus influenzae]
ATGACTTATAAACTCATTTTTGATAAACGCGCATTAAAAGAATGGAATAAATTGGGGGAAACCTTACGCCAACAATTTAA
AAATAAATTAGCCGAAAGAATGATAAATCCACGCATTCAAGCCGACAAATTAAAGGGCGAAAATGATTTATATAAAATCA
AATTAAGAAGCGCTGGTTATCGATTAGTTTATCAAGTAAGAGATCAGGAAATCACTATTATTGTTGTATCTGTTGGAAAA
AGGGAACGATTAGAAGTCTATAAAACCGCAGAACAAAGAACATCCCACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9419.78 Da
• Isoelectric Point: 4.0737

>AT32168 WP_005651514.1 NZ_AP018775:75454-75708 [Haemophilus influenzae]
MALHQILAEQAASITDLKRNPMGVIQESESGIVAILNRNQPAFYCITPELFSHMQELIEDLELGRMANERLATLEPVEVD
LNDL

Nucleotide

• Length: 255 bp

>AT32168 NZ_AP018775:75454-75708 [Haemophilus influenzae]
ATGGCATTACATCAAATACTGGCAGAACAAGCGGCAAGCATTACAGACTTAAAACGAAACCCAATGGGCGTAATTCAAGA
AAGCGAAAGCGGAATAGTCGCAATATTGAACCGAAACCAACCCGCATTTTATTGCATCACCCCCGAATTATTCTCTCATA
TGCAAGAATTAATCGAAGATTTAGAGCTCGGCAGAATGGCAAATGAACGGCTAGCCACCCTAGAACCAGTAGAGGTTGAT
CTAAATGACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A5C2QYX4