Detailed information of TA system TA032162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 368411..369059
• Replicon: chromosome
• Accession: NZ_AP018774
• Organism: Haemophilus influenzae strain CHBN-III-3 isolate 13H42

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A0Y7JUP0
• Locus tag: K6J70_RS01815
• Protein ID: WP_005694481.1
• Coordinates: 368411..368770 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: K6J70_RS01820
• Protein ID: WP_005650837.1
• Coordinates: 368763..369059 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J70_RS01810 (CHBNIII3_03500)	364878..368141	+	3264	WP_275426380.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
K6J70_RS01815 (CHBNIII3_03510)	368411..368770	+	360	WP_005694481.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6J70_RS01820 (CHBNIII3_03520)	368763..369059	+	297	WP_005650837.1	helix-turn-helix transcriptional regulator	Antitoxin
K6J70_RS01825 (CHBNIII3_03530)	369176..371092	+	1917	WP_012054642.1	ABC transporter ATP-binding protein	-
K6J70_RS01830 (CHBNIII3_03540)	371102..371638	+	537	WP_012054643.1	type I DNA topoisomerase	-
K6J70_RS01835 (CHBNIII3_03550)	371654..372205	+	552	WP_012054644.1	Sua5/YciO/YrdC/YwlC family protein	-
K6J70_RS01840 (CHBNIII3_03560)	372209..373015	+	807	WP_012054645.1	shikimate dehydrogenase	-
K6J70_RS01845 (CHBNIII3_03570)	373027..373785	+	759	WP_012054646.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (36-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 14321.67 Da
• Isoelectric Point: 10.1408

>T32162 WP_005694481.1 NZ_AP018774:368411-368770 [Haemophilus influenzae]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVKLLSELGTSVGKPYVKHLDGEIWELRPIRDRILFARLM
DGRFVLLHQFMKKTQKTPKREIQTAQQRLSELKERLKNE

Nucleotide

• Length: 360 bp

>T32162 NZ_AP018774:368411-368770 [Haemophilus influenzae]
ATGTACGAGATTCTCTTTTACAGAGACCAAAATGACATTGAACCAGTGAAAGAGTATCTGCTATCGCTGGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAAAGTTATTAAGCGAACTCGGGACGTCTGTTGGCA
AACCCTATGTGAAACATTTAGACGGTGAAATTTGGGAACTTCGTCCAATTAGGGATCGCATCTTGTTTGCAAGATTAATG
GACGGTCGCTTTGTCTTACTACATCAATTTATGAAGAAAACACAAAAAACGCCAAAACGAGAAATCCAAACTGCCCAACA
ACGCTTAAGTGAATTAAAAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11092.94 Da
• Isoelectric Point: 10.3139

>AT32162 WP_005650837.1 NZ_AP018774:368763-369059 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide

• Length: 297 bp

>AT32162 NZ_AP018774:368763-369059 [Haemophilus influenzae]
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGCGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAGCAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAACTTGAAACGTTATTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGGGTAAAAAATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0Y7JUP0