Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1674926..1675515 Replicon chromosome
Accession NZ_AP018773
Organism Haemophilus influenzae strain CHBN-III-2 isolate 13H39

Toxin (Protein)


Gene name hicA Uniprot ID Q4QL51
Locus tag K6J68_RS08340 Protein ID WP_005653200.1
Coordinates 1675267..1675515 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q4QL50
Locus tag K6J68_RS08335 Protein ID WP_005653201.1
Coordinates 1674926..1675270 (-) Length 115 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J68_RS08320 (CHBNIII2_15860) 1670149..1671543 - 1395 WP_005657016.1 sodium-coupled multidrug efflux MATE transporter HmrM -
K6J68_RS08325 (CHBNIII2_15870) 1671587..1672201 + 615 WP_005657019.1 riboflavin synthase -
K6J68_RS08330 (CHBNIII2_15880) 1672273..1674882 - 2610 WP_012054428.1 aminopeptidase N -
K6J68_RS08335 (CHBNIII2_15890) 1674926..1675270 - 345 WP_005653201.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K6J68_RS08340 (CHBNIII2_15900) 1675267..1675515 - 249 WP_005653200.1 type II toxin-antitoxin system HicA family toxin Toxin
K6J68_RS08345 (CHBNIII2_15910) 1675686..1676180 + 495 WP_005653194.1 5-(carboxyamino)imidazole ribonucleotide mutase -
K6J68_RS08350 (CHBNIII2_15920) 1676250..1677338 + 1089 WP_005686592.1 5-(carboxyamino)imidazole ribonucleotide synthase -
K6J68_RS08355 (CHBNIII2_15930) 1677478..1678668 + 1191 WP_005657030.1 amino acid aminotransferase -
K6J68_RS08360 (CHBNIII2_15940) 1678775..1679401 - 627 WP_011272462.1 energy-coupling factor ABC transporter ATP-binding protein -
K6J68_RS08365 (CHBNIII2_15950) 1679403..1680035 - 633 WP_011272461.1 energy-coupling factor transporter transmembrane protein EcfT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-102)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9425.93 Da        Isoelectric Point: 10.4673

>T32157 WP_005653200.1 NZ_AP018773:c1675515-1675267 [Haemophilus influenzae]
MGRTEKLLDKLAQSKSTFNWNELVSLLAQQGYEKREMAGSRVRFYNRTLEHTILLHKPHPENYIKGGALKSVKESLKQVG
IL

Download         Length: 249 bp

>T32157 NZ_AP018773:c1675515-1675267 [Haemophilus influenzae]
ATGGGACGAACTGAAAAATTATTAGATAAGCTCGCACAATCAAAATCTACATTTAATTGGAATGAATTAGTTTCTTTGTT
AGCTCAACAAGGTTATGAAAAGCGAGAAATGGCAGGCTCTCGAGTGAGATTTTATAATAGAACACTCGAACATACGATTT
TGTTACACAAGCCTCACCCTGAAAATTATATTAAAGGTGGTGCTTTAAAGTCAGTGAAAGAATCATTAAAACAGGTAGGT
ATTCTATGA

Antitoxin


Download         Length: 115 a.a.        Molecular weight: 13142.04 Da        Isoelectric Point: 4.8151

>AT32157 WP_005653201.1 NZ_AP018773:c1675270-1674926 [Haemophilus influenzae]
MKLLNYKGYVGTIEADLENNILFGKLAYIRDLVTYEAESLSELEKEFRQSVDLYLQDCLELGKEPNKPFKGVFNVRIGEE
LHREATIIAGDRSLNAFVTEAIQEKIFREKPSLR

Download         Length: 345 bp

>AT32157 NZ_AP018773:c1675270-1674926 [Haemophilus influenzae]
ATGAAGTTATTAAATTATAAAGGTTATGTTGGCACGATTGAGGCGGATTTAGAAAACAATATATTGTTTGGCAAACTTGC
TTACATTCGTGATTTAGTGACTTACGAAGCAGAGTCATTATCTGAGCTAGAAAAAGAATTTCGTCAATCTGTTGATTTAT
ATTTACAAGATTGTTTGGAATTAGGTAAAGAACCGAATAAGCCTTTTAAAGGTGTATTTAATGTACGAATTGGCGAGGAA
TTGCATAGAGAAGCTACGATCATAGCAGGCGATCGTTCTCTTAATGCTTTTGTTACGGAAGCGATTCAAGAAAAAATTTT
TCGTGAAAAACCAAGTTTAAGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5M6BP03

Antitoxin

Source ID Structure
AlphaFold DB A0A0M1GMF9

References