Detailed information of TA system TA032152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 368764..369235
• Replicon: chromosome
• Accession: NZ_AP018773
• Organism: Haemophilus influenzae strain CHBN-III-2 isolate 13H39

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A806DTA9
• Locus tag: K6J68_RS01830
• Protein ID: WP_005654546.1
• Coordinates: 368764..368946 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: K6J68_RS01835
• Protein ID: WP_005650837.1
• Coordinates: 368939..369235 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J68_RS01820	365010..365174	-	165	WP_012054638.1	hypothetical protein	-
K6J68_RS01825 (CHBNIII2_03510)	365477..368494	+	3018	WP_221281815.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
K6J68_RS01830 (CHBNIII2_03520)	368764..368946	+	183	WP_005654546.1	shikimate 5-dehydrogenase	Toxin
K6J68_RS01835 (CHBNIII2_03530)	368939..369235	+	297	WP_005650837.1	helix-turn-helix transcriptional regulator	Antitoxin
K6J68_RS01840 (CHBNIII2_03540)	369419..371335	+	1917	WP_105197700.1	ABC transporter ATP-binding protein	-
K6J68_RS01845 (CHBNIII2_03550)	371345..371881	+	537	WP_105197698.1	type I DNA topoisomerase	-
K6J68_RS01850 (CHBNIII2_03560)	371897..372448	+	552	WP_105197696.1	Sua5/YciO/YrdC/YwlC family protein	-
K6J68_RS01855 (CHBNIII2_03570)	372452..373258	+	807	WP_105197694.1	shikimate dehydrogenase	-
K6J68_RS01860 (CHBNIII2_03580)	373270..374028	+	759	WP_012054646.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7318.33 Da
• Isoelectric Point: 6.3057

>T32152 WP_005654546.1 NZ_AP018773:368764-368946 [Haemophilus influenzae]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVIQTAQQRLSELKERLKNE

Nucleotide

• Length: 183 bp

>T32152 NZ_AP018773:368764-368946 [Haemophilus influenzae]
ATGTACGAGATTCTCTTTTACAGAGACCAAAATGACATTGAGCCAGTGAAAGAATATCTGCTATCGCTGGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAATCCAAACAGCCCAACAACGCTTAAGTGAATTAA
AAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11092.94 Da
• Isoelectric Point: 10.3139

>AT32152 WP_005650837.1 NZ_AP018773:368939-369235 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide

• Length: 297 bp

>AT32152 NZ_AP018773:368939-369235 [Haemophilus influenzae]
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGCGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAACAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAACTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGGGTAAAAAATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A806DTA9