Detailed information of TA system TA032147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1577043..1577632
• Replicon: chromosome
• Accession: NZ_AP018772
• Organism: Haemophilus influenzae strain CHBN-III-1 isolate 15H02

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q709A1
• Locus tag: K6J58_RS07645
• Protein ID: WP_005686594.1
• Coordinates: 1577384..1577632 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q9R3K0
• Locus tag: K6J58_RS07640
• Protein ID: WP_005686595.1
• Coordinates: 1577043..1577387 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J58_RS07630 (CHBNIII1_14500)	1573356..1573970	+	615	WP_005657019.1	riboflavin synthase	-
K6J58_RS07635 (CHBNIII1_14510)	1574042..1576651	-	2610	WP_005686596.1	aminopeptidase N	-
K6J58_RS07640 (CHBNIII1_14520)	1577043..1577387	-	345	WP_005686595.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K6J58_RS07645 (CHBNIII1_14530)	1577384..1577632	-	249	WP_005686594.1	type II toxin-antitoxin system HicA family toxin	Toxin
K6J58_RS07650 (CHBNIII1_14540)	1577803..1578297	+	495	WP_005686593.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
K6J58_RS07655 (CHBNIII1_14550)	1578367..1579455	+	1089	WP_005686592.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
K6J58_RS07660 (CHBNIII1_14560)	1579595..1580785	+	1191	WP_005686591.1	amino acid aminotransferase	-
K6J58_RS07665 (CHBNIII1_14570)	1580890..1581516	-	627	WP_005686589.1	energy-coupling factor ABC transporter ATP-binding protein	-
K6J58_RS07670 (CHBNIII1_14580)	1581518..1582150	-	633	WP_005686586.1	energy-coupling factor transporter transmembrane protein EcfT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-102)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9396.93 Da
• Isoelectric Point: 10.7140

>T32147 WP_005686594.1 NZ_AP018772:c1577632-1577384 [Haemophilus influenzae]
MGRTEKLLDKLAQSKSTFNWNELASLLAQQGYEKREMAGSRVRFYNRTLKHTILLHKPHPENYIKGGALKSVKESLKQVG
IL

Nucleotide

• Length: 249 bp

>T32147 NZ_AP018772:c1577632-1577384 [Haemophilus influenzae]
ATGGGACGAACTGAAAAATTATTAGATAAGCTCGCACAATCAAAATCTACATTTAATTGGAATGAATTAGCTTCTTTGTT
AGCTCAACAAGGTTATGAAAAGCGAGAAATGGCAGGCTCTCGAGTGAGATTTTATAATAGAACACTCAAGCACACGATTT
TGTTACACAAGCCTCACCCTGAAAATTATATTAAAGGCGGTGCTTTAAAGTCAGTGAAAGAATCATTAAAACAGGTAGGT
ATTCTATGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13123.03 Da
• Isoelectric Point: 5.0042

>AT32147 WP_005686595.1 NZ_AP018772:c1577387-1577043 [Haemophilus influenzae]
MKLLNYKGYVGTIEADLENNILFGKLAYIRDLVTYEAESLSELEKEFHQSVDLYLQDCLELGKEPNKPFKGVFNVRIGEE
LHREATIIAGDRSLNAFVTEAIKEKIFREKPSLR

Nucleotide

• Length: 345 bp

>AT32147 NZ_AP018772:c1577387-1577043 [Haemophilus influenzae]
ATGAAGTTATTAAATTATAAAGGTTATGTTGGCACGATTGAGGCGGATTTAGAAAACAATATATTATTTGGCAAACTTGC
TTACATTCGTGATTTAGTAACTTACGAAGCAGAGTCATTATCTGAGTTAGAAAAAGAATTTCATCAATCTGTTGATTTAT
ATTTACAAGATTGTTTGGAATTAGGTAAAGAACCGAATAAGCCTTTTAAAGGTGTATTTAATGTACGAATTGGCGAGGAA
TTGCATAGAGAAGCAACGATCATAGCTGGCGATCGTTCTCTTAATGCTTTTGTGACGGAAGCAATTAAAGAAAAAATTTT
TCGTGAAAAACCAAGTTTAAGATAA

Structures

Toxin

• AlphaFold DB: Q709A1

Antitoxin

• AlphaFold DB: Q9R3K0