Detailed information of TA system TA032143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 369500..369971
• Replicon: chromosome
• Accession: NZ_AP018772
• Organism: Haemophilus influenzae strain CHBN-III-1 isolate 15H02

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A806DTA9
• Locus tag: K6J58_RS01825
• Protein ID: WP_005654546.1
• Coordinates: 369500..369682 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: K6J58_RS01830
• Protein ID: WP_005650837.1
• Coordinates: 369675..369971 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J58_RS08840	366236..366379	+	144	WP_246589444.1	PT domain-containing protein	-
K6J58_RS01820 (CHBNIII1_03540)	366326..369230	+	2905	Protein_356	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
K6J58_RS01825 (CHBNIII1_03550)	369500..369682	+	183	WP_005654546.1	shikimate 5-dehydrogenase	Toxin
K6J58_RS01830 (CHBNIII1_03560)	369675..369971	+	297	WP_005650837.1	helix-turn-helix transcriptional regulator	Antitoxin
K6J58_RS01835 (CHBNIII1_03570)	370155..372071	+	1917	WP_005688147.1	ABC transporter ATP-binding protein	-
K6J58_RS01840 (CHBNIII1_03580)	372081..372617	+	537	WP_005688146.1	type I DNA topoisomerase	-
K6J58_RS01845 (CHBNIII1_03590)	372633..373184	+	552	WP_005688145.1	Sua5/YciO/YrdC/YwlC family protein	-
K6J58_RS01850 (CHBNIII1_03600)	373188..373994	+	807	WP_005688144.1	shikimate dehydrogenase	-
K6J58_RS01855 (CHBNIII1_03610)	374006..374797	+	792	WP_005688143.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7318.33 Da
• Isoelectric Point: 6.3057

>T32143 WP_005654546.1 NZ_AP018772:369500-369682 [Haemophilus influenzae]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVIQTAQQRLSELKERLKNE

Nucleotide

• Length: 183 bp

>T32143 NZ_AP018772:369500-369682 [Haemophilus influenzae]
ATGTACGAAATTCTCTTTTACAGAGACCAAAATGACATTGAACCAGTGAAAGAGTATCTGCTATCGCTGGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAATCCAAACTGCCCAACAACGCTTAAGTGAATTAA
AAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11092.94 Da
• Isoelectric Point: 10.3139

>AT32143 WP_005650837.1 NZ_AP018772:369675-369971 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide

• Length: 297 bp

>AT32143 NZ_AP018772:369675-369971 [Haemophilus influenzae]
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGTCAGCAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAACTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGGGTAAAAAATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A806DTA9