Detailed information of TA system TA032139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 45753..46384
• Replicon: chromosome
• Accession: NZ_AP018772
• Organism: Haemophilus influenzae strain CHBN-III-1 isolate 15H02

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q4QLW0
• Locus tag: K6J58_RS00225
• Protein ID: WP_005651560.1
• Coordinates: 45986..46384 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K6J58_RS00220
• Protein ID: WP_005686209.1
• Coordinates: 45753..45986 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J58_RS00195 (CHBNIII1_00400)	41232..42434	-	1203	WP_005686217.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
K6J58_RS00200 (CHBNIII1_00410)	42598..43263	+	666	WP_032821549.1	DNA repair protein RadC	-
K6J58_RS00205 (CHBNIII1_00420)	43477..43713	+	237	WP_005542826.1	50S ribosomal protein L28	-
K6J58_RS00210 (CHBNIII1_00430)	43725..43895	+	171	WP_005613503.1	50S ribosomal protein L33	-
K6J58_RS00215 (CHBNIII1_00440)	44242..45606	+	1365	WP_005686211.1	diaminobutyrate--2-oxoglutarate transaminase	-
K6J58_RS00220 (CHBNIII1_00450)	45753..45986	+	234	WP_005686209.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
K6J58_RS00225 (CHBNIII1_00460)	45986..46384	+	399	WP_005651560.1	tRNA(fMet)-specific endonuclease VapC	Toxin
K6J58_RS00230 (CHBNIII1_00470)	46404..47939	+	1536	WP_005686206.1	L-2,4-diaminobutyrate decarboxylase	-
K6J58_RS00235 (CHBNIII1_00480)	48160..48975	+	816	WP_005686204.1	DNA-formamidopyrimidine glycosylase	-
K6J58_RS00240 (CHBNIII1_00490)	49049..50071	-	1023	WP_005655937.1	outer membrane-stress sensor serine endopeptidase DegS	-
K6J58_RS00245 (CHBNIII1_00500)	50072..51190	-	1119	WP_005686201.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15115.46 Da
• Isoelectric Point: 8.0514

>T32139 WP_005651560.1 NZ_AP018772:45986-46384 [Haemophilus influenzae]
VLKYMLDTNIVIYVIKRRPLEILSRFNQNAGKMCVSSITVAELYYGAEKSEYPERNIAVIEDFLSRLTILDYQPKHAAHF
GNIKAELSKQGKLIGENDIHIAAHARSEGLILVSNNLREFERVIALRTENWV

Nucleotide

• Length: 399 bp

>T32139 NZ_AP018772:45986-46384 [Haemophilus influenzae]
GTGTTGAAATATATGCTTGATACTAACATTGTGATTTACGTGATTAAGCGTAGGCCATTGGAAATTCTTTCTCGCTTTAA
TCAAAATGCAGGAAAAATGTGCGTGAGTAGCATTACTGTGGCTGAACTCTATTATGGAGCTGAAAAAAGCGAATATCCCG
AAAGAAACATTGCGGTTATTGAAGATTTCTTATCACGCCTCACCATTTTAGATTACCAACCAAAGCATGCAGCTCATTTC
GGCAATATTAAAGCTGAACTCTCTAAACAAGGGAAATTGATTGGCGAAAATGATATTCATATAGCGGCTCATGCTCGTAG
TGAAGGGTTAATTCTGGTTAGTAACAATTTAAGAGAATTTGAGCGAGTTATAGCATTACGTACAGAAAATTGGGTGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8911.01 Da
• Isoelectric Point: 4.4013

>AT32139 WP_005686209.1 NZ_AP018772:45753-45986 [Haemophilus influenzae]
MIETSVFMTNRSQAVRLPAEVRFSEEIKKLSVRVSGSDRILSPLNQSWDSFFLNDQAVSDDFMNEREIAFQPEREAL

Nucleotide

• Length: 234 bp

>AT32139 NZ_AP018772:45753-45986 [Haemophilus influenzae]
ATGATTGAAACCTCTGTTTTTATGACTAATCGAAGTCAAGCTGTGCGTTTGCCCGCTGAAGTTCGCTTTTCTGAAGAAAT
CAAAAAATTATCGGTTCGTGTATCGGGCAGTGATCGGATTTTATCGCCTCTCAACCAATCTTGGGATAGTTTTTTCTTGA
ATGATCAAGCAGTTAGCGATGATTTTATGAATGAACGAGAAATTGCATTTCAACCAGAGCGTGAGGCTTTATAG

Structures

Toxin

• AlphaFold DB: Q4QLW0