Detailed information of TA system TA032136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1283657..1284301
• Replicon: chromosome
• Accession: NZ_AP018771
• Organism: Haemophilus influenzae strain CHBN-II-8 isolate 10H27

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q4QKY0
• Locus tag: K6J66_RS06280
• Protein ID: WP_005643265.1
• Coordinates: 1283657..1283842 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K6J66_RS06285
• Protein ID: WP_105899288.1
• Coordinates: 1283873..1284301 (+)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J66_RS06265 (CHBNII8_11830)	1278779..1280023	-	1245	WP_005657489.1	tyrosine-type recombinase/integrase	-
K6J66_RS06270 (CHBNII8_11840)	1280793..1281803	-	1011	WP_110442646.1	phage portal protein	-
K6J66_RS06275 (CHBNII8_11850)	1281813..1283588	-	1776	WP_110442647.1	terminase family protein	-
K6J66_RS06280	1283657..1283842	+	186	WP_005643265.1	type II toxin-antitoxin system HicA family toxin	Toxin
K6J66_RS06285 (CHBNII8_11860)	1283873..1284301	+	429	WP_105899288.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K6J66_RS06290	1284393..1284572	-	180	WP_005672190.1	hypothetical protein	-
K6J66_RS06295 (CHBNII8_11870)	1284566..1285198	+	633	WP_110442648.1	BRO family protein	-
K6J66_RS06300 (CHBNII8_11880)	1285376..1286182	+	807	WP_110442649.1	GPO family capsid scaffolding protein	-
K6J66_RS06305 (CHBNII8_11890)	1286204..1287253	+	1050	WP_110442650.1	phage major capsid protein, P2 family	-
K6J66_RS06310 (CHBNII8_11900)	1287265..1287915	+	651	WP_110442651.1	phage terminase small subunit	-
K6J66_RS06315 (CHBNII8_11910)	1288209..1288715	+	507	WP_110442652.1	head completion/stabilization protein	-
K6J66_RS06320 (CHBNII8_11920)	1288715..1288924	+	210	WP_021035391.1	tail protein X	-
K6J66_RS06325 (CHBNII8_11930)	1288926..1289147	+	222	WP_105891216.1	phage holin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1272897..1317204	44307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-135)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6716.88 Da
• Isoelectric Point: 11.1595

>T32136 WP_005643265.1 NZ_AP018771:1283657-1283842 [Haemophilus influenzae]
MRSSDLIKELKSAGCTFVRHGKGDHQIWQSPITGKTFPVPHPKQHVPIGTLRSIKKSAGLL

Nucleotide

• Length: 186 bp

>T32136 NZ_AP018771:1283657-1283842 [Haemophilus influenzae]
ATGCGATCCAGCGACTTAATCAAGGAACTTAAAAGTGCAGGTTGTACTTTTGTCAGGCACGGTAAAGGCGATCATCAAAT
CTGGCAATCGCCCATCACAGGGAAGACGTTCCCCGTACCACACCCAAAACAACACGTACCCATCGGCACATTAAGATCCA
TTAAAAAATCGGCAGGGCTTCTATAG

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 16444.43 Da
• Isoelectric Point: 4.3241

>AT32136 WP_105899288.1 NZ_AP018771:1283873-1284301 [Haemophilus influenzae]
MLFTIGIETPTNENEAYGITVPALFTEEYSCFSAADTLEEIPTQVTDAIHSILEMMFEDGLDINELQDKGYRHYQTQEDF
NYCDTWLLLDVDISAYQGKRHRINISLPEYLIKRIDSRVASNPIYKDRSHFLAVASQKELRQ

Nucleotide

• Length: 429 bp

>AT32136 NZ_AP018771:1283873-1284301 [Haemophilus influenzae]
ATGTTATTCACTATTGGCATTGAAACCCCAACAAACGAAAATGAAGCCTACGGCATCACTGTGCCAGCATTATTTACTGA
AGAATATTCCTGCTTTAGTGCCGCTGATACCCTTGAAGAAATCCCAACGCAGGTAACTGATGCCATTCATTCCATCTTAG
AAATGATGTTTGAAGACGGCTTAGATATCAACGAACTTCAAGACAAAGGCTATCGTCACTACCAAACGCAAGAAGATTTC
AACTATTGCGATACTTGGTTGTTGCTTGATGTAGATATTTCCGCATATCAAGGCAAACGCCACCGTATTAATATCAGCTT
GCCTGAATACCTTATCAAACGTATTGATAGCCGTGTTGCAAGCAACCCCATTTACAAAGACCGCAGCCATTTTTTAGCAG
TTGCTTCACAAAAAGAGCTACGGCAATAA

Structures

Toxin

• AlphaFold DB: Q4QKY0