Detailed information of TA system TA032134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 690635..691272
• Replicon: chromosome
• Accession: NZ_AP018771
• Organism: Haemophilus influenzae strain CHBN-II-8 isolate 10H27

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: -
• Locus tag: K6J66_RS03455
• Protein ID: WP_229380845.1
• Coordinates: 690635..691030 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: A0A0H3PN67
• Locus tag: K6J66_RS03460
• Protein ID: WP_005656316.1
• Coordinates: 691036..691272 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J66_RS03425 (CHBNII8_06520)	686190..686756	+	567	WP_005662944.1	elongation factor P	-
K6J66_RS03435 (CHBNII8_06530)	687112..687699	-	588	WP_038439260.1	primosomal replication protein	-
K6J66_RS03440 (CHBNII8_06540)	687732..689084	-	1353	WP_038439259.1	Na+/H+ antiporter family protein	-
K6J66_RS03445 (CHBNII8_06550)	689398..690087	-	690	WP_005659978.1	ribonuclease T	-
K6J66_RS03450 (CHBNII8_06560)	690161..690568	-	408	WP_005628548.1	lactoylglutathione lyase	-
K6J66_RS03455 (CHBNII8_06570)	690635..691030	-	396	WP_229380845.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6J66_RS03460 (CHBNII8_06580)	691036..691272	-	237	WP_005656316.1	antitoxin	Antitoxin
K6J66_RS03465 (CHBNII8_06590)	691365..692090	-	726	WP_038439255.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
K6J66_RS03470 (CHBNII8_06600)	692143..692661	-	519	WP_038439252.1	isoprenylcysteine carboxyl methyltransferase family protein	-
K6J66_RS03475 (CHBNII8_06610)	692881..694647	+	1767	WP_038439250.1	aspartate--tRNA ligase	-
K6J66_RS03480 (CHBNII8_06620)	694669..695142	+	474	WP_038439249.1	dihydroneopterin triphosphate diphosphatase	-
K6J66_RS03485 (CHBNII8_06630)	695154..695894	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-121)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15229.68 Da
• Isoelectric Point: 8.4762

>T32134 WP_229380845.1 NZ_AP018771:c691030-690635 [Haemophilus influenzae]
MLDTNIIIYLMKNRPKIVAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLHYGK
WASTLKKQGQPIGNNDLWIACHALSLNAVLITHNIKEFQRITDLQWQDWTK

Nucleotide

• Length: 396 bp

>T32134 NZ_AP018771:c691030-690635 [Haemophilus influenzae]
ATGTTAGACACTAATATCATTATTTATTTAATGAAAAATCGTCCCAAAATTGTTGCCGAACGAGTATCACAATTATTGCC
TAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCGTTTGGTAGTCAAAATTATGAGCAAT
CAATACGAGCAATAGAATTGCTTACTGAGCGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACATTATGGCAAA
TGGGCAAGTACACTCAAAAAACAGGGGCAACCTATCGGAAATAATGACCTATGGATTGCTTGCCACGCATTGAGTTTAAA
TGCTGTTCTTATTACACATAATATAAAAGAGTTTCAGCGTATTACAGATCTTCAATGGCAAGATTGGACAAAATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9007.16 Da
• Isoelectric Point: 4.2342

>AT32134 WP_005656316.1 NZ_AP018771:c691272-691036 [Haemophilus influenzae]
MLAKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT32134 NZ_AP018771:c691272-691036 [Haemophilus influenzae]
ATGCTTGCTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGATGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGAGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3PN67