Detailed information of TA system TA032129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 749978..750615
• Replicon: chromosome
• Accession: NZ_AP018770
• Organism: Haemophilus influenzae strain CHBN-II-7 isolate 13H35

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: K6J57_RS03825
• Protein ID: WP_005649049.1
• Coordinates: 749978..750382 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: K6J57_RS03830
• Protein ID: WP_005649046.1
• Coordinates: 750379..750615 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J57_RS03795 (CHBNII7_07260)	745538..746104	+	567	WP_005544066.1	elongation factor P	-
K6J57_RS03805 (CHBNII7_07270)	746455..747042	-	588	WP_221266041.1	primosomal replication protein	-
K6J57_RS03810 (CHBNII7_07280)	747075..748427	-	1353	WP_005694344.1	Na+/H+ antiporter family protein	-
K6J57_RS03815 (CHBNII7_07290)	748741..749430	-	690	WP_011271988.1	ribonuclease T	-
K6J57_RS03820 (CHBNII7_07300)	749504..749911	-	408	WP_005688980.1	lactoylglutathione lyase	-
K6J57_RS03825 (CHBNII7_07310)	749978..750382	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6J57_RS03830 (CHBNII7_07320)	750379..750615	-	237	WP_005649046.1	antitoxin	Antitoxin
K6J57_RS03835 (CHBNII7_07330)	750708..751433	-	726	WP_011271987.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
K6J57_RS03840 (CHBNII7_07340)	751486..752004	-	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
K6J57_RS03845 (CHBNII7_07350)	752223..753989	+	1767	WP_042594297.1	aspartate--tRNA ligase	-
K6J57_RS03850 (CHBNII7_07360)	754011..754484	+	474	WP_011271985.1	dihydroneopterin triphosphate diphosphatase	-
K6J57_RS03855 (CHBNII7_07370)	754496..755236	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T32129 WP_005649049.1 NZ_AP018770:c750382-749978 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T32129 NZ_AP018770:c750382-749978 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT32129 WP_005649046.1 NZ_AP018770:c750615-750379 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT32129 NZ_AP018770:c750615-750379 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3