Detailed information of TA system TA032123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 695239..695876
• Replicon: chromosome
• Accession: NZ_AP018769
• Organism: Haemophilus influenzae strain CHBN-II-6 isolate 12H01

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: K6J61_RS03470
• Protein ID: WP_005649049.1
• Coordinates: 695239..695643 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: K6J61_RS03475
• Protein ID: WP_005649046.1
• Coordinates: 695640..695876 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J61_RS03440 (CHBNII6_06540)	690799..691365	+	567	WP_005544066.1	elongation factor P	-
K6J61_RS03450 (CHBNII6_06550)	691716..692303	-	588	WP_005654063.1	primosomal replication protein	-
K6J61_RS03455 (CHBNII6_06560)	692336..693688	-	1353	WP_005694344.1	Na+/H+ antiporter family protein	-
K6J61_RS03460 (CHBNII6_06570)	694002..694691	-	690	WP_011271988.1	ribonuclease T	-
K6J61_RS03465 (CHBNII6_06580)	694765..695172	-	408	WP_005688980.1	lactoylglutathione lyase	-
K6J61_RS03470 (CHBNII6_06590)	695239..695643	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6J61_RS03475 (CHBNII6_06600)	695640..695876	-	237	WP_005649046.1	antitoxin	Antitoxin
K6J61_RS03480 (CHBNII6_06610)	695969..696694	-	726	WP_011271987.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
K6J61_RS03485 (CHBNII6_06620)	696747..697265	-	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
K6J61_RS03490 (CHBNII6_06630)	697484..699250	+	1767	WP_110459383.1	aspartate--tRNA ligase	-
K6J61_RS03495 (CHBNII6_06640)	699272..699745	+	474	WP_011271985.1	dihydroneopterin triphosphate diphosphatase	-
K6J61_RS03500 (CHBNII6_06650)	699757..700497	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T32123 WP_005649049.1 NZ_AP018769:c695643-695239 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T32123 NZ_AP018769:c695643-695239 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT32123 WP_005649046.1 NZ_AP018769:c695876-695640 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT32123 NZ_AP018769:c695876-695640 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3