Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) VapXD/-
Location 575306..575784 Replicon chromosome
Accession NZ_AP018769
Organism Haemophilus influenzae strain CHBN-II-6 isolate 12H01

Toxin (Protein)


Gene name VapD Uniprot ID Q4QN95
Locus tag K6J61_RS02840 Protein ID WP_011272058.1
Coordinates 575506..575784 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name VapX Uniprot ID Q4QN94
Locus tag K6J61_RS02835 Protein ID WP_011272059.1
Coordinates 575306..575497 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J61_RS02815 (CHBNII6_05320) 571514..572497 + 984 WP_011272062.1 DNA polymerase III subunit delta' -
K6J61_RS02820 (CHBNII6_05330) 572552..573334 + 783 WP_011272061.1 YchF/TatD family DNA exonuclease -
K6J61_RS02825 (CHBNII6_05340) 573361..573885 + 525 WP_011272060.1 DUF1523 family protein -
K6J61_RS02830 (CHBNII6_05350) 573936..575234 + 1299 WP_005691371.1 hemolysin family protein -
K6J61_RS02835 (CHBNII6_05360) 575306..575497 + 192 WP_011272059.1 DUF5397 family protein Antitoxin
K6J61_RS02840 (CHBNII6_05370) 575506..575784 + 279 WP_011272058.1 virulence-associated protein VapD Toxin
K6J61_RS02845 (CHBNII6_05380) 575877..576407 + 531 WP_005649345.1 hypothetical protein -
K6J61_RS02850 (CHBNII6_05390) 576479..577978 + 1500 WP_110459370.1 fused PTS fructose transporter subunit IIA/HPr protein -
K6J61_RS02855 (CHBNII6_05400) 577980..578921 + 942 WP_011272055.1 1-phosphofructokinase -
K6J61_RS02860 (CHBNII6_05410) 578923..580593 + 1671 WP_011272054.1 fructose-specific PTS transporter subunit EIIC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Virulence-associated protein D / CRISPR associated protein Cas2

(1-86)

Antitoxin

Protein of unknown function DUF5397

(1-63)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10647.08 Da        Isoelectric Point: 4.9670

>T32122 WP_011272058.1 NZ_AP018769:575506-575784 [Haemophilus influenzae]
MYAIAFDLVVKDTQDYHPKGVQEAYTDIGAVLAKFGFVRTQGSLYTNMNEDMANLFQAMNALKQLAWISQSVRDIRAFRI
EQWSDFTDFIRN

Download         Length: 279 bp

>T32122 NZ_AP018769:575506-575784 [Haemophilus influenzae]
ATGTACGCGATTGCTTTTGATTTAGTTGTAAAAGACACTCAAGATTATCATCCAAAGGGCGTTCAAGAGGCTTATACAGA
CATTGGCGCAGTTTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATACCAACATGAATGAAGATATGGCGA
ATCTCTTTCAGGCAATGAATGCACTAAAACAGTTGGCGTGGATTTCTCAGTCGGTACGAGATATTCGCGCTTTTCGTATT
GAGCAATGGTCTGATTTTACTGATTTTATACGAAATTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7073.06 Da        Isoelectric Point: 3.8431

>AT32122 WP_011272059.1 NZ_AP018769:575306-575497 [Haemophilus influenzae]
MELRQQIPTGCIKQFGQFGVPYVVGEVAEFLPDGDVLVNITLLQSGEKDIYRLSYLLEDPEAE

Download         Length: 192 bp

>AT32122 NZ_AP018769:575306-575497 [Haemophilus influenzae]
ATGGAACTAAGACAACAAATTCCAACAGGCTGTATTAAGCAATTCGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGTGATGTGTTGGTAAATATCACATTGTTGCAATCTGGAGAAAAAGACATTTATCGCTTAT
CTTACCTACTTGAAGATCCCGAAGCTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6ZI0
PDB 6ZN8
PDB 6ZI1

Antitoxin

Source ID Structure
PDB 6ZN8
AlphaFold DB Q4QN94

References