Detailed information of TA system TA032121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 375712..376228
• Replicon: chromosome
• Accession: NZ_AP018769
• Organism: Haemophilus influenzae strain CHBN-II-6 isolate 12H01

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A806DTA9
• Locus tag: K6J61_RS01825
• Protein ID: WP_005654546.1
• Coordinates: 375712..375894 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: K6J61_RS01830
• Protein ID: WP_105893707.1
• Coordinates: 375887..376228 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J61_RS01820 (CHBNII6_03410)	372371..375442	+	3072	WP_221252646.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
K6J61_RS01825 (CHBNII6_03420)	375712..375894	+	183	WP_005654546.1	shikimate 5-dehydrogenase	Toxin
K6J61_RS01830 (CHBNII6_03430)	375887..376228	+	342	WP_105893707.1	helix-turn-helix transcriptional regulator	Antitoxin
K6J61_RS01835 (CHBNII6_03440)	376351..378267	+	1917	WP_110459250.1	ABC transporter ATP-binding protein	-
K6J61_RS01840 (CHBNII6_03450)	378277..378825	+	549	WP_048942741.1	type I DNA topoisomerase	-
K6J61_RS01845 (CHBNII6_03460)	378841..379392	+	552	WP_048942742.1	Sua5/YciO/YrdC/YwlC family protein	-
K6J61_RS01850 (CHBNII6_03470)	379396..380202	+	807	WP_110459251.1	shikimate dehydrogenase	-
K6J61_RS01855 (CHBNII6_03480)	380416..380979	+	564	WP_110459252.1	DNA-3-methyladenine glycosylase I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7318.33 Da
• Isoelectric Point: 6.3057

>T32121 WP_005654546.1 NZ_AP018769:375712-375894 [Haemophilus influenzae]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVIQTAQQRLSELKERLKNE

Nucleotide

• Length: 183 bp

>T32121 NZ_AP018769:375712-375894 [Haemophilus influenzae]
ATGTACGAGATTCTCTTTTACAGAGACCAAAATGACATTGAGCCAGTGAAAGAGTATCTGCTATCGCTGGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAATCCAAACTGCCCAACAACGCTTAAGTGAATTAA
AAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13106.39 Da
• Isoelectric Point: 9.8470

>AT32121 WP_105893707.1 NZ_AP018769:375887-376228 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKKCLRYTHCFHIDLLYYK

Nucleotide

• Length: 342 bp

>AT32121 NZ_AP018769:375887-376228 [Haemophilus influenzae]
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAATTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGCGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAACAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAACTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGGGTAAAAAAATGCCTAAGATATACCCATTGTTTTCATAT
TGACTTGTTATATTATAAATAG

Structures

Toxin

• AlphaFold DB: A0A806DTA9