Detailed information of TA system TA032116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 689875..690512
• Replicon: chromosome
• Accession: NZ_AP018768
• Organism: Haemophilus influenzae strain CHBN-II-5 isolate 13H40

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: K6J56_RS03480
• Protein ID: WP_005649049.1
• Coordinates: 689875..690279 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: K6J56_RS03485
• Protein ID: WP_005649046.1
• Coordinates: 690276..690512 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J56_RS03450 (CHBNII5_06540)	685435..686001	+	567	WP_005544066.1	elongation factor P	-
K6J56_RS03460 (CHBNII5_06550)	686352..686939	-	588	WP_005654063.1	primosomal replication protein	-
K6J56_RS03465 (CHBNII5_06560)	686972..688324	-	1353	WP_005694344.1	Na+/H+ antiporter family protein	-
K6J56_RS03470 (CHBNII5_06570)	688638..689327	-	690	WP_011271988.1	ribonuclease T	-
K6J56_RS03475 (CHBNII5_06580)	689401..689808	-	408	WP_005688980.1	lactoylglutathione lyase	-
K6J56_RS03480 (CHBNII5_06590)	689875..690279	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6J56_RS03485 (CHBNII5_06600)	690276..690512	-	237	WP_005649046.1	antitoxin	Antitoxin
K6J56_RS03490 (CHBNII5_06610)	690605..691330	-	726	WP_011271987.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
K6J56_RS03495 (CHBNII5_06620)	691383..691901	-	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
K6J56_RS03500 (CHBNII5_06630)	692120..693886	+	1767	WP_011271986.1	aspartate--tRNA ligase	-
K6J56_RS03505 (CHBNII5_06640)	693908..694381	+	474	WP_011271985.1	dihydroneopterin triphosphate diphosphatase	-
K6J56_RS03510 (CHBNII5_06650)	694393..695133	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T32116 WP_005649049.1 NZ_AP018768:c690279-689875 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T32116 NZ_AP018768:c690279-689875 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT32116 WP_005649046.1 NZ_AP018768:c690512-690276 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT32116 NZ_AP018768:c690512-690276 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3