Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) VapXD/-
Location 569828..570306 Replicon chromosome
Accession NZ_AP018768
Organism Haemophilus influenzae strain CHBN-II-5 isolate 13H40

Toxin (Protein)


Gene name VapD Uniprot ID -
Locus tag K6J56_RS02845 Protein ID WP_044364768.1
Coordinates 570028..570306 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name VapX Uniprot ID Q4QN94
Locus tag K6J56_RS02840 Protein ID WP_011272059.1
Coordinates 569828..570019 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J56_RS02815 (CHBNII5_05320) 565407..566039 + 633 WP_044365145.1 dTMP kinase -
K6J56_RS02820 (CHBNII5_05330) 566036..567019 + 984 WP_044364765.1 DNA polymerase III subunit delta' -
K6J56_RS02825 (CHBNII5_05340) 567074..567856 + 783 WP_011272061.1 YchF/TatD family DNA exonuclease -
K6J56_RS02830 (CHBNII5_05350) 567883..568407 + 525 WP_011272060.1 DUF1523 family protein -
K6J56_RS02835 (CHBNII5_05360) 568458..569756 + 1299 WP_005691371.1 hemolysin family protein -
K6J56_RS02840 (CHBNII5_05370) 569828..570019 + 192 WP_011272059.1 DUF5397 family protein Antitoxin
K6J56_RS02845 (CHBNII5_05380) 570028..570306 + 279 WP_044364768.1 virulence-associated protein VapD Toxin
K6J56_RS02850 (CHBNII5_05390) 570399..570929 + 531 WP_005649345.1 hypothetical protein -
K6J56_RS02855 (CHBNII5_05400) 571001..572500 + 1500 WP_011272056.1 fused PTS fructose transporter subunit IIA/HPr protein -
K6J56_RS02860 (CHBNII5_05410) 572502..573443 + 942 WP_011272055.1 1-phosphofructokinase -
K6J56_RS02865 (CHBNII5_05420) 573445..575115 + 1671 WP_011272054.1 fructose-specific PTS transporter subunit EIIC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Virulence-associated protein D / CRISPR associated protein Cas2

(1-86)

Antitoxin

Protein of unknown function DUF5397

(1-63)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10598.99 Da        Isoelectric Point: 4.6712

>T32115 WP_044364768.1 NZ_AP018768:570028-570306 [Haemophilus influenzae]
MYAIAFDLVVKDTQDDHPKGVQEAYTDIGAVLAKFGFVRTQGSLYTNMNEDMANLFQAMNALKQLAWISQSVRDIRAFRI
EQWSDFTDFIRN

Download         Length: 279 bp

>T32115 NZ_AP018768:570028-570306 [Haemophilus influenzae]
ATGTACGCGATTGCTTTTGATTTAGTTGTAAAAGACACTCAAGATGATCATCCAAAGGGCGTTCAAGAGGCTTATACAGA
CATTGGCGCAGTTTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATACCAACATGAATGAAGATATGGCGA
ATCTCTTTCAGGCAATGAATGCACTAAAACAGTTGGCGTGGATTTCTCAGTCGGTACGAGATATTCGCGCTTTTCGTATT
GAGCAATGGTCTGATTTTACTGATTTTATACGAAATTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7073.06 Da        Isoelectric Point: 3.8431

>AT32115 WP_011272059.1 NZ_AP018768:569828-570019 [Haemophilus influenzae]
MELRQQIPTGCIKQFGQFGVPYVVGEVAEFLPDGDVLVNITLLQSGEKDIYRLSYLLEDPEAE

Download         Length: 192 bp

>AT32115 NZ_AP018768:569828-570019 [Haemophilus influenzae]
ATGGAACTAAGACAACAAATTCCAACAGGCTGTATTAAGCAATTCGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGTGATGTGTTGGTAAATATCACATTGTTACAATCTGGAGAAAAAGACATTTATCGCTTAT
CTTACCTACTTGAAGATCCCGAAGCTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6ZN8
AlphaFold DB Q4QN94

References