Detailed information of TA system TA032109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 705549..706186
• Replicon: chromosome
• Accession: NZ_AP018767
• Organism: Haemophilus influenzae strain CHBN-II-4 isolate 13H33

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: K6J55_RS03530
• Protein ID: WP_005649049.1
• Coordinates: 705549..705953 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: K6J55_RS03535
• Protein ID: WP_005649046.1
• Coordinates: 705950..706186 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J55_RS03500 (CHBNII4_06620)	701109..701675	+	567	WP_005544066.1	elongation factor P	-
K6J55_RS03510 (CHBNII4_06630)	702026..702613	-	588	WP_005654063.1	primosomal replication protein	-
K6J55_RS03515 (CHBNII4_06640)	702646..703998	-	1353	WP_005694344.1	Na+/H+ antiporter family protein	-
K6J55_RS03520 (CHBNII4_06650)	704312..705001	-	690	WP_011271988.1	ribonuclease T	-
K6J55_RS03525 (CHBNII4_06660)	705075..705482	-	408	WP_005688980.1	lactoylglutathione lyase	-
K6J55_RS03530 (CHBNII4_06670)	705549..705953	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6J55_RS03535 (CHBNII4_06680)	705950..706186	-	237	WP_005649046.1	antitoxin	Antitoxin
K6J55_RS03540 (CHBNII4_06690)	706279..707004	-	726	WP_011271987.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
K6J55_RS03545 (CHBNII4_06700)	707057..707575	-	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
K6J55_RS03550 (CHBNII4_06710)	707794..709560	+	1767	WP_042594297.1	aspartate--tRNA ligase	-
K6J55_RS03555 (CHBNII4_06720)	709582..710055	+	474	WP_011271985.1	dihydroneopterin triphosphate diphosphatase	-
K6J55_RS03560 (CHBNII4_06730)	710067..710807	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T32109 WP_005649049.1 NZ_AP018767:c705953-705549 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T32109 NZ_AP018767:c705953-705549 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT32109 WP_005649046.1 NZ_AP018767:c706186-705950 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT32109 NZ_AP018767:c706186-705950 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3