Detailed information of TA system TA032101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 705628..706265
• Replicon: chromosome
• Accession: NZ_AP018766
• Organism: Haemophilus influenzae strain CHBN-II-3 isolate 13H41

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: K6J54_RS03520
• Protein ID: WP_005649049.1
• Coordinates: 705628..706032 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: K6J54_RS03525
• Protein ID: WP_005649046.1
• Coordinates: 706029..706265 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J54_RS03490 (CHBNII3_06650)	701186..701752	+	567	WP_005662944.1	elongation factor P	-
K6J54_RS03500 (CHBNII3_06660)	702104..702691	-	588	WP_005654063.1	primosomal replication protein	-
K6J54_RS03505 (CHBNII3_06670)	702724..704076	-	1353	WP_005688977.1	Na+/H+ antiporter family protein	-
K6J54_RS03510 (CHBNII3_06680)	704391..705080	-	690	WP_005688979.1	ribonuclease T	-
K6J54_RS03515 (CHBNII3_06690)	705154..705561	-	408	WP_005688980.1	lactoylglutathione lyase	-
K6J54_RS03520 (CHBNII3_06700)	705628..706032	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6J54_RS03525 (CHBNII3_06710)	706029..706265	-	237	WP_005649046.1	antitoxin	Antitoxin
K6J54_RS03530 (CHBNII3_06720)	706358..707083	-	726	WP_061724197.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
K6J54_RS03535 (CHBNII3_06730)	707136..707654	-	519	WP_061724196.1	isoprenylcysteine carboxyl methyltransferase family protein	-
K6J54_RS03540 (CHBNII3_06740)	707873..709639	+	1767	WP_221265041.1	aspartate--tRNA ligase	-
K6J54_RS03545 (CHBNII3_06750)	709701..710573	+	873	WP_005688989.1	hypothetical protein	-
K6J54_RS09210	710776..711057	-	282	Protein_682	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T32101 WP_005649049.1 NZ_AP018766:c706032-705628 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T32101 NZ_AP018766:c706032-705628 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT32101 WP_005649046.1 NZ_AP018766:c706265-706029 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT32101 NZ_AP018766:c706265-706029 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3