Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) VapXD/-
Location 644944..645422 Replicon chromosome
Accession NZ_AP018765
Organism Haemophilus influenzae strain CHBN-II-2 isolate 12H49

Toxin (Protein)


Gene name VapD Uniprot ID Q4QN95
Locus tag K6J63_RS03315 Protein ID WP_011272058.1
Coordinates 645144..645422 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name VapX Uniprot ID Q4QN94
Locus tag K6J63_RS03310 Protein ID WP_011272059.1
Coordinates 644944..645135 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J63_RS03290 (CHBNII2_06320) 641154..642137 + 984 WP_105892797.1 DNA polymerase III subunit delta' -
K6J63_RS03295 (CHBNII2_06330) 642190..642972 + 783 WP_038440581.1 YchF/TatD family DNA exonuclease -
K6J63_RS03300 (CHBNII2_06340) 642999..643523 + 525 WP_038440582.1 DUF1523 family protein -
K6J63_RS03305 (CHBNII2_06350) 643574..644872 + 1299 WP_005691371.1 hemolysin family protein -
K6J63_RS03310 (CHBNII2_06360) 644944..645135 + 192 WP_011272059.1 DUF5397 family protein Antitoxin
K6J63_RS03315 (CHBNII2_06370) 645144..645422 + 279 WP_011272058.1 virulence-associated protein VapD Toxin
K6J63_RS03320 (CHBNII2_06380) 645515..646045 + 531 WP_038440586.1 hypothetical protein -
K6J63_RS03325 (CHBNII2_06390) 646117..647616 + 1500 WP_011272056.1 fused PTS fructose transporter subunit IIA/HPr protein -
K6J63_RS03330 (CHBNII2_06400) 647618..648559 + 942 WP_110418007.1 1-phosphofructokinase -
K6J63_RS03335 (CHBNII2_06410) 648561..650231 + 1671 WP_011272054.1 fructose-specific PTS transporter subunit EIIC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Virulence-associated protein D / CRISPR associated protein Cas2

(1-86)

Antitoxin

Protein of unknown function DUF5397

(1-63)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10647.08 Da        Isoelectric Point: 4.9670

>T32093 WP_011272058.1 NZ_AP018765:645144-645422 [Haemophilus influenzae]
MYAIAFDLVVKDTQDYHPKGVQEAYTDIGAVLAKFGFVRTQGSLYTNMNEDMANLFQAMNALKQLAWISQSVRDIRAFRI
EQWSDFTDFIRN

Download         Length: 279 bp

>T32093 NZ_AP018765:645144-645422 [Haemophilus influenzae]
ATGTACGCGATTGCTTTTGATTTAGTTGTAAAAGACACTCAAGATTATCATCCAAAGGGCGTTCAAGAGGCTTATACAGA
CATTGGCGCAGTTTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATACCAACATGAATGAAGATATGGCGA
ATCTCTTTCAGGCAATGAATGCACTAAAACAGTTGGCGTGGATTTCTCAGTCGGTACGAGATATTCGCGCTTTTCGTATT
GAGCAATGGTCTGATTTTACTGATTTTATACGAAATTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7073.06 Da        Isoelectric Point: 3.8431

>AT32093 WP_011272059.1 NZ_AP018765:644944-645135 [Haemophilus influenzae]
MELRQQIPTGCIKQFGQFGVPYVVGEVAEFLPDGDVLVNITLLQSGEKDIYRLSYLLEDPEAE

Download         Length: 192 bp

>AT32093 NZ_AP018765:644944-645135 [Haemophilus influenzae]
ATGGAACTAAGACAACAAATTCCAACAGGCTGTATTAAGCAATTCGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGTGATGTGTTGGTAAATATCACATTGTTACAATCTGGAGAAAAAGACATTTATCGCTTAT
CTTACCTACTTGAAGATCCCGAAGCTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6ZI0
PDB 6ZN8
PDB 6ZI1

Antitoxin

Source ID Structure
PDB 6ZN8
AlphaFold DB Q4QN94

References