Detailed information of TA system TA032088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 814223..814860
• Replicon: chromosome
• Accession: NZ_AP018764
• Organism: Haemophilus influenzae strain CHBN-II-1 isolate 13H34

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: K6J65_RS04265
• Protein ID: WP_005649049.1
• Coordinates: 814223..814627 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: K6J65_RS04270
• Protein ID: WP_005649046.1
• Coordinates: 814624..814860 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J65_RS04235 (CHBNII1_08190)	809783..810349	+	567	WP_005544066.1	elongation factor P	-
K6J65_RS04245 (CHBNII1_08200)	810700..811287	-	588	WP_005654063.1	primosomal replication protein	-
K6J65_RS04250 (CHBNII1_08210)	811320..812672	-	1353	WP_005694344.1	Na+/H+ antiporter family protein	-
K6J65_RS04255 (CHBNII1_08220)	812986..813675	-	690	WP_011271988.1	ribonuclease T	-
K6J65_RS04260 (CHBNII1_08230)	813749..814156	-	408	WP_005688980.1	lactoylglutathione lyase	-
K6J65_RS04265 (CHBNII1_08240)	814223..814627	-	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6J65_RS04270 (CHBNII1_08250)	814624..814860	-	237	WP_005649046.1	antitoxin	Antitoxin
K6J65_RS04275 (CHBNII1_08260)	814953..815678	-	726	WP_105892704.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
K6J65_RS04280 (CHBNII1_08270)	815731..816249	-	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
K6J65_RS04285 (CHBNII1_08280)	816468..818234	+	1767	WP_011271986.1	aspartate--tRNA ligase	-
K6J65_RS04290 (CHBNII1_08290)	818256..818729	+	474	WP_011271985.1	dihydroneopterin triphosphate diphosphatase	-
K6J65_RS04295 (CHBNII1_08300)	818741..819481	+	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T32088 WP_005649049.1 NZ_AP018764:c814627-814223 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T32088 NZ_AP018764:c814627-814223 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT32088 WP_005649046.1 NZ_AP018764:c814860-814624 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT32088 NZ_AP018764:c814860-814624 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3