Detailed information of TA system TA032084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 147773..148299
• Replicon: plasmid pMRY16-398_1
• Accession: NZ_AP018757
• Organism: Metakosakonia sp. MRY16-398

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: MRY16398_RS29755
• Protein ID: WP_000323025.1
• Coordinates: 148012..148299 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J5W3H0
• Locus tag: MRY16398_RS29750
• Protein ID: WP_004196370.1
• Coordinates: 147773..148012 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRY16398_RS29720	143110..143302	-	193	Protein_156	MFS transporter	-
MRY16398_RS29725	143332..144309	+	978	WP_004196334.1	chromate resistance protein	-
MRY16398_RS29730	144266..145642	+	1377	WP_004196363.1	chromate efflux transporter	-
MRY16398_RS29735	145673..146362	-	690	WP_004196322.1	hypothetical protein	-
MRY16398_RS29740	146376..147113	-	738	WP_008460272.1	HupE/UreJ family protein	-
MRY16398_RS29745	147157..147522	-	366	WP_009651956.1	hypothetical protein	-
MRY16398_RS29750	147773..148012	+	240	WP_004196370.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
MRY16398_RS29755	148012..148299	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
MRY16398_RS29760	148371..148529	+	159	WP_014063856.1	type I toxin-antitoxin system Hok family toxin	-
MRY16398_RS29765	148734..148988	-	255	WP_125125755.1	DUF2767 family protein	-
MRY16398_RS29770	149555..150133	+	579	WP_125125756.1	hypothetical protein	-
MRY16398_RS29775	150205..150507	+	303	WP_125125757.1	hypothetical protein	-
MRY16398_RS29780	151233..151535	+	303	WP_047368814.1	hypothetical protein	-
MRY16398_RS29785	151549..152367	+	819	WP_172600722.1	DUF932 domain-containing protein	-
MRY16398_RS29790	152384..152929	-	546	WP_125125759.1	lytic transglycosylase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..224544	224544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T32084 WP_000323025.1 NZ_AP018757:148012-148299 [Metakosakonia sp. MRY16-398]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T32084 NZ_AP018757:148012-148299 [Metakosakonia sp. MRY16-398]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9018.43 Da
• Isoelectric Point: 4.3894

>AT32084 WP_004196370.1 NZ_AP018757:147773-148012 [Metakosakonia sp. MRY16-398]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT32084 NZ_AP018757:147773-148012 [Metakosakonia sp. MRY16-398]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A2J4QRH4