Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 82053..82696 Replicon plasmid pMRY16-398_1
Accession NZ_AP018757
Organism Metakosakonia sp. MRY16-398

Toxin (Protein)


Gene name vagD Uniprot ID A0A348DMH6
Locus tag MRY16398_RS29390 Protein ID WP_047368852.1
Coordinates 82053..82469 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A348DMH7
Locus tag MRY16398_RS29395 Protein ID WP_047368851.1
Coordinates 82466..82696 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRY16398_RS29380 78828..79070 + 243 WP_047368854.1 hypothetical protein -
MRY16398_RS29385 80637..81866 + 1230 WP_047368853.1 HDOD domain-containing protein -
MRY16398_RS29390 82053..82469 - 417 WP_047368852.1 type II toxin-antitoxin system VapC family toxin Toxin
MRY16398_RS29395 82466..82696 - 231 WP_047368851.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MRY16398_RS29400 83041..83301 + 261 WP_007374405.1 type II toxin-antitoxin system ParD family antitoxin -
MRY16398_RS29405 83288..83584 + 297 WP_047368850.1 type II toxin-antitoxin system RelE/ParE family toxin -
MRY16398_RS29410 83814..84248 - 435 WP_007374421.1 hypothetical protein -
MRY16398_RS29415 84466..85866 - 1401 WP_007374420.1 copper resistance membrane spanning protein PcoS -
MRY16398_RS29420 85863..86543 - 681 WP_001188930.1 copper response regulator transcription factor PcoR -
MRY16398_RS29425 86598..87527 - 930 WP_000998778.1 copper resistance inner membrane protein PcoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..224544 224544


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15042.32 Da        Isoelectric Point: 7.2449

>T32083 WP_047368852.1 NZ_AP018757:c82469-82053 [Metakosakonia sp. MRY16-398]
VNKTYMLDTNICSLIMREQPEAVLKRLEQAVLRNHRTVISAITYQEMRSGATGPKASPRHIALVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAVAAGAILVTNNTREFERVPGLVLEDWVN

Download         Length: 417 bp

>T32083 NZ_AP018757:c82469-82053 [Metakosakonia sp. MRY16-398]
GTGAACAAAACGTATATGCTGGACACTAACATCTGCTCATTAATCATGCGCGAGCAACCGGAAGCGGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCAACCACCGCACTGTGATCTCGGCCATCACCTACCAGGAGATGCGCTCCGGCGCCACGGGCC
CGAAGGCTTCGCCGCGCCATATTGCGCTGGTTGATGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCG
GCGGTCGACGCTACCACGGACATCCGGGTGGCGCTGCGCCTCGCAGGCACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCATGCGGTTGCCGCCGGCGCCATCCTGGTGACCAACAATACAAGAGAGTTTGAACGGGTTCCGGGTCTGGTGCTGG
AAGACTGGGTAAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8780.97 Da        Isoelectric Point: 4.6583

>AT32083 WP_047368851.1 NZ_AP018757:c82696-82466 [Metakosakonia sp. MRY16-398]
MRTVSIFKNGNNRAIRLPRDMDFEGVSELEIVREGDRIILRPVRPTWGSFAKMEKADPDFMAEREDVVSDEGRVDL

Download         Length: 231 bp

>AT32083 NZ_AP018757:c82696-82466 [Metakosakonia sp. MRY16-398]
ATGAGAACCGTATCTATTTTCAAAAACGGCAACAACCGCGCCATCCGTCTGCCCCGTGACATGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGAGACCGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGAAGATGG
AAAAAGCAGACCCGGACTTTATGGCGGAGCGTGAGGACGTTGTCAGTGACGAAGGACGGGTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A348DMH6

Antitoxin

Source ID Structure
AlphaFold DB A0A348DMH7

References