Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4529772..4530391 Replicon chromosome
Accession NZ_AP018756
Organism Metakosakonia sp. MRY16-398

Toxin (Protein)


Gene name Hha Uniprot ID A0A348DII6
Locus tag MRY16398_RS22250 Protein ID WP_041852465.1
Coordinates 4530173..4530391 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A348DII5
Locus tag MRY16398_RS22245 Protein ID WP_041852464.1
Coordinates 4529772..4530146 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRY16398_RS22235 4524883..4526079 + 1197 WP_041852462.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MRY16398_RS22240 4526102..4529251 + 3150 WP_125125202.1 multidrug efflux RND transporter permease subunit AcrB -
MRY16398_RS22245 4529772..4530146 + 375 WP_041852464.1 Hha toxicity modulator TomB Antitoxin
MRY16398_RS22250 4530173..4530391 + 219 WP_041852465.1 hemolysin expression modulator Hha Toxin
MRY16398_RS22255 4530460..4531026 + 567 WP_125125203.1 maltose O-acetyltransferase -
MRY16398_RS22260 4531126..4531593 + 468 WP_041852467.1 membrane protein -
MRY16398_RS22265 4531571..4533016 - 1446 WP_125125204.1 PLP-dependent aminotransferase family protein -
MRY16398_RS22270 4533116..4533817 + 702 WP_041852469.1 GNAT family N-acetyltransferase -
MRY16398_RS22275 4533814..4533954 - 141 WP_006818844.1 type B 50S ribosomal protein L36 -
MRY16398_RS22280 4533958..4534218 - 261 WP_041852470.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8685.14 Da        Isoelectric Point: 8.9039

>T32081 WP_041852465.1 NZ_AP018756:4530173-4530391 [Metakosakonia sp. MRY16-398]
MSDKPLTKIDYLMRLRRCQTIDTLERVIEKNKYELPDHELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T32081 NZ_AP018756:4530173-4530391 [Metakosakonia sp. MRY16-398]
ATGTCTGATAAGCCATTAACCAAAATTGATTATTTGATGCGATTACGACGTTGTCAGACAATTGACACGCTCGAACGCGT
TATTGAAAAAAATAAATACGAACTGCCTGACCATGAACTGACGGTGTTTTATTCAGCAGCCGATCATCGTCTGGCTGAGC
TAACCATGAATAAACTGTATGACAAAATCCCTCCTTCTGTGTGGAAATTCGTACGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14600.44 Da        Isoelectric Point: 5.5072

>AT32081 WP_041852464.1 NZ_AP018756:4529772-4530146 [Metakosakonia sp. MRY16-398]
MDEYSPKRHDIAQLKFLCETLYHDCLLNLEESHHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNVSKANPVSHSC

Download         Length: 375 bp

>AT32081 NZ_AP018756:4529772-4530146 [Metakosakonia sp. MRY16-398]
ATGGACGAATACTCGCCAAAAAGGCATGATATCGCGCAGCTTAAATTTCTCTGCGAAACGTTGTACCATGATTGTCTCCT
GAATCTTGAAGAAAGCCATCATGGATGGGTTAACGATCCTACTTCGGCAATCAATTTGCAGTTAAATGAGTTGATTGAAC
ATATAGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAAGATAATAAACTGATTGAGCAGATAGACGAATACCTT
GATGATACGTTTATGCTGTTCAGTAGCTACGGTATCAACACACAAGACTTGCAGAAATGGCGTAAATCGGGAAACCGATT
GTTCCGCTGTTTCGTCAATGTGAGCAAGGCTAACCCTGTTAGTCACTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A348DII6

Antitoxin

Source ID Structure
AlphaFold DB A0A348DII5

References