Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 2952659..2953302 Replicon chromosome
Accession NZ_AP018756
Organism Metakosakonia sp. MRY16-398

Toxin (Protein)


Gene name vagD Uniprot ID A0A348DE83
Locus tag MRY16398_RS14555 Protein ID WP_071194208.1
Coordinates 2952886..2953302 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A348DE82
Locus tag MRY16398_RS14550 Protein ID WP_071194209.1
Coordinates 2952659..2952889 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRY16398_RS14530 2948821..2949198 + 378 WP_108701294.1 VOC family protein -
MRY16398_RS14535 2949269..2950222 + 954 WP_039079008.1 2-hydroxyacid dehydrogenase -
MRY16398_RS14540 2950274..2951038 + 765 WP_039079009.1 SDR family oxidoreductase -
MRY16398_RS14545 2951115..2952401 - 1287 WP_125124672.1 MFS transporter -
MRY16398_RS14550 2952659..2952889 + 231 WP_071194209.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MRY16398_RS14555 2952886..2953302 + 417 WP_071194208.1 PIN domain-containing protein Toxin
MRY16398_RS14560 2953565..2953972 - 408 WP_125125677.1 nuclear transport factor 2 family protein -
MRY16398_RS14565 2954257..2955255 + 999 WP_039079015.1 ABC transporter substrate-binding protein -
MRY16398_RS14570 2955255..2956292 + 1038 WP_125124673.1 iron ABC transporter permease -
MRY16398_RS14575 2956292..2957053 + 762 WP_071194204.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15050.57 Da        Isoelectric Point: 9.2345

>T32071 WP_071194208.1 NZ_AP018756:2952886-2953302 [Metakosakonia sp. MRY16-398]
MKKTFMLDTNICSFIMREHPESVIKRLEQAVLRNNRIVVSAITYAEMRFGAIGKKASPHHAKLVDAFCARLDAVLAWDRA
AVDATTQIKLALALAGTPIGPNDTAIAGHAIAAGVVLVTNNTREFERVPGLILEDWAK

Download         Length: 417 bp

>T32071 NZ_AP018756:2952886-2953302 [Metakosakonia sp. MRY16-398]
ATGAAGAAAACCTTTATGCTCGACACTAATATTTGCTCATTCATCATGCGCGAGCATCCGGAAAGCGTTATTAAGCGTCT
GGAGCAGGCAGTGTTGCGCAACAATCGAATCGTAGTATCGGCTATCACCTATGCGGAAATGCGCTTCGGCGCGATCGGTA
AAAAGGCGTCGCCGCATCATGCGAAGCTGGTTGATGCGTTCTGTGCTCGCCTTGATGCCGTGCTCGCCTGGGACCGGGCG
GCAGTGGATGCCACTACGCAAATAAAGCTTGCGCTGGCGTTGGCTGGTACGCCGATTGGTCCGAACGATACCGCGATTGC
CGGGCATGCTATTGCCGCTGGCGTCGTGCTGGTAACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTGATTCTGG
AAGACTGGGCAAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8783.91 Da        Isoelectric Point: 4.3654

>AT32071 WP_071194209.1 NZ_AP018756:2952659-2952889 [Metakosakonia sp. MRY16-398]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIIREGDCIILRPVRPTWSSFAQLEKADPDFMAEREDVVSDEGRFDL

Download         Length: 231 bp

>AT32071 NZ_AP018756:2952659-2952889 [Metakosakonia sp. MRY16-398]
ATGCGAACCGTATCCATCTTCAAAAACGGCAACAATCGTGCCATCCGTCTGCCTCGCGATCTGGATTTCGAAGGAGTGAG
CGAACTGGAGATCATCAGGGAAGGGGACTGCATCATCCTGCGCCCGGTGCGACCGACGTGGAGTTCTTTCGCGCAATTAG
AAAAAGCCGACCCGGATTTTATGGCAGAACGTGAAGATGTGGTCAGTGATGAGGGACGCTTTGATCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A348DE83

Antitoxin

Source ID Structure
AlphaFold DB A0A348DE82

References