Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 201219..201958 Replicon chromosome
Accession NZ_AP018756
Organism Metakosakonia sp. MRY16-398

Toxin (Protein)


Gene name tacT Uniprot ID A0A348D6L8
Locus tag MRY16398_RS00975 Protein ID WP_039080181.1
Coordinates 201473..201958 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A348D6L7
Locus tag MRY16398_RS00970 Protein ID WP_039080180.1
Coordinates 201219..201485 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRY16398_RS00945 197291..197596 + 306 WP_172600614.1 YsaB family lipoprotein -
MRY16398_RS00950 197702..198613 + 912 WP_004126458.1 glycine--tRNA ligase subunit alpha -
MRY16398_RS00955 198623..200692 + 2070 WP_039080179.1 glycine--tRNA ligase subunit beta -
MRY16398_RS00960 200813..201061 + 249 Protein_189 IS30 family transposase -
MRY16398_RS00970 201219..201485 + 267 WP_039080180.1 DUF1778 domain-containing protein Antitoxin
MRY16398_RS00975 201473..201958 + 486 WP_039080181.1 GNAT family N-acetyltransferase Toxin
MRY16398_RS00980 202544..204163 + 1620 WP_107222628.1 ATP-binding cassette domain-containing protein -
MRY16398_RS00985 204207..204500 - 294 WP_039080183.1 HTH-type transcriptional regulator -
MRY16398_RS00990 204878..205588 + 711 WP_039080184.1 DUF3053 domain-containing protein -
MRY16398_RS00995 205654..206628 - 975 WP_071195041.1 glyoxylate/hydroxypyruvate reductase GhrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17775.56 Da        Isoelectric Point: 10.2934

>T32066 WP_039080181.1 NZ_AP018756:201473-201958 [Metakosakonia sp. MRY16-398]
VGRVTAPEPLSNFHQVAAFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKKDTKQIVGFYSLATGSVNHTETTGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T32066 NZ_AP018756:201473-201958 [Metakosakonia sp. MRY16-398]
GTGGGACGTGTAACAGCGCCAGAGCCTTTATCCAATTTCCATCAGGTGGCGGCGTTTGTTAGCGGTGAAGCTGTGCTCGA
TGATTGGCTAAAGCAGAGAGGCCTCAAAAATCAGGCTCTCGGAGCTGCCCGAACTTTTGTTGTATGTAAGAAAGACACGA
AACAAATAGTCGGTTTTTACTCTTTGGCCACCGGTAGCGTCAACCATACAGAAACTACAGGCAACCTTCGGCGTAACATG
CCAGACCCTATTCCTGTGATTATACTCGCCCGGCTTGCTGTCGACGTCTCCTTTCGTGGAAAAGGGCTTGGCGCTGATTT
ACTCCACGATGCGGTGCTTCGTTGTTATCGAGTTGCTGAAAATATTGGTGTGCGTGCAATCATGGTTCACGCGCTGACGG
AAGAAGCCAAAAATTTCTATATTCACCATGGTTTCAAGCCATCACAAACTCAGCAGCGGACGTTATTCCTTAAACTTCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT32066 WP_039080180.1 NZ_AP018756:201219-201485 [Metakosakonia sp. MRY16-398]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILERRAFNFNDEQYAEFIDMLDAPVADDPVIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT32066 NZ_AP018756:201219-201485 [Metakosakonia sp. MRY16-398]
ATGAAATCAGATGTTCAACTTAACCTTAGAGCCAAGGAATCACAGCGAGCACTCATTGATGCCGCCGCTGAGATCCTGCA
CAAATCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCTGAAAATGTGATTCTTGAGCGACGTGCCTTTAATT
TTAACGATGAGCAATATGCAGAGTTCATCGATATGCTCGATGCACCGGTTGCGGATGATCCCGTTATCGACAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A348D6L8

Antitoxin

Source ID Structure
AlphaFold DB A0A348D6L7

References