Detailed information of TA system TA032065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 27590..28233
• Replicon: plasmid pKP6702
• Accession: NZ_AP018755
• Organism: Klebsiella pneumoniae strain KP67

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: D5075_RS28615
• Protein ID: WP_001044770.1
• Coordinates: 27590..28006 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: D5075_RS28620
• Protein ID: WP_001261282.1
• Coordinates: 28003..28233 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5075_RS28585	22793..23581	+	789	WP_001206315.1	ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1	-
D5075_RS28590	23641..24465	+	825	WP_000722315.1	oxacillin-hydrolyzing class D beta-lactamase OXA-9	-
D5075_RS28595	24665..24982	+	318	Protein_28	recombinase family protein	-
D5075_RS28600	25165..26025	+	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-
D5075_RS28605	26075..26772	-	698	Protein_30	IS1 family transposase	-
D5075_RS28610	26806..27516	-	711	WP_004152334.1	ATP-binding protein	-
D5075_RS28615	27590..28006	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
D5075_RS28620	28003..28233	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D5075_RS29470	28190..28651	+	462	WP_072202616.1	hypothetical protein	-
D5075_RS28630	28795..29145	+	351	WP_000493378.1	hypothetical protein	-
D5075_RS28635	29196..29939	+	744	WP_000129823.1	hypothetical protein	-
D5075_RS28640	29936..30712	+	777	WP_000015958.1	site-specific integrase	-
D5075_RS28645	30770..31027	-	258	WP_000764642.1	hypothetical protein	-
D5075_RS29475	31156..31260	-	105	WP_032409716.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-15 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A	-	1..31833	31833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T32065 WP_001044770.1 NZ_AP018755:c28006-27590 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T32065 NZ_AP018755:c28006-27590 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT32065 WP_001261282.1 NZ_AP018755:c28233-28003 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT32065 NZ_AP018755:c28233-28003 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3