32027

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 161027..161549 Replicon plasmid pKP3301
Accession NZ_AP018748
Organism Klebsiella pneumoniae strain KP33

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag D5079_RS27155 Protein ID WP_038991638.1
Coordinates 161265..161549 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag D5079_RS27150 Protein ID WP_004181777.1
Coordinates 161027..161275 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D5079_RS27130 156236..157057 - 822 WP_004181772.1 hypothetical protein -
D5079_RS27135 157119..157472 - 354 WP_004181774.1 hypothetical protein -
D5079_RS27140 157617..158603 - 987 WP_040120328.1 hypothetical protein -
D5079_RS27145 158937..160736 - 1800 WP_043907032.1 ATP-dependent helicase -
D5079_RS27150 161027..161275 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D5079_RS27155 161265..161549 + 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D5079_RS27160 161566..161667 - 102 Protein_168 IS200/IS605 family transposase -
D5079_RS27165 161703..162929 + 1227 WP_080030933.1 transposase -
D5079_RS27170 163201..163425 - 225 Protein_170 transposase -
D5079_RS27175 163504..163932 - 429 WP_077256229.1 IS200/IS605 family transposase -
D5079_RS27180 163968..165155 + 1188 WP_043907031.1 transposase -
D5079_RS27185 165254..166349 - 1096 Protein_173 conjugal transfer protein TraG N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / aac(6')-Ib-cr / blaOXA-1 - 1..296750 296750


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T32027 WP_038991638.1 NZ_AP018748:161265-161549 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T32027 NZ_AP018748:161265-161549 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT32027 WP_004181777.1 NZ_AP018748:161027-161275 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT32027 NZ_AP018748:161027-161275 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References