Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 82886..83637 Replicon plasmid pKP3301
Accession NZ_AP018748
Organism Klebsiella pneumoniae strain KP33

Toxin (Protein)


Gene name ataT Uniprot ID H6U1U8
Locus tag D5079_RS26675 Protein ID WP_014386536.1
Coordinates 82886..83368 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A071LPN3
Locus tag D5079_RS26680 Protein ID WP_004902250.1
Coordinates 83359..83637 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D5079_RS26645 79285..79932 - 648 WP_014386537.1 EcsC family protein -
D5079_RS26650 79959..80714 - 756 WP_004902235.1 DUF2971 domain-containing protein -
D5079_RS26655 80815..81207 - 393 WP_032442757.1 hypothetical protein -
D5079_RS26660 81312..81851 - 540 WP_004902239.1 hypothetical protein -
D5079_RS26675 82886..83368 - 483 WP_014386536.1 GNAT family N-acetyltransferase Toxin
D5079_RS26680 83359..83637 - 279 WP_004902250.1 DUF1778 domain-containing protein Antitoxin
D5079_RS26685 83756..83968 - 213 WP_004902255.1 hypothetical protein -
D5079_RS26690 84076..84417 - 342 WP_004902257.1 hypothetical protein -
D5079_RS26700 85247..85705 - 459 WP_014386535.1 hypothetical protein -
D5079_RS26705 86358..86813 - 456 WP_001166628.1 Hg(II)-responsive transcriptional regulator -
D5079_RS26710 86885..87250 + 366 WP_004200999.1 mercuric ion transporter MerT -
D5079_RS26715 87266..87541 + 276 WP_000732275.1 mercury resistance system periplasmic binding protein MerP -
D5079_RS26720 87569..87994 + 426 WP_000522996.1 organomercurial transporter MerC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / aac(6')-Ib-cr / blaOXA-1 - 1..296750 296750


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(10-86)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17662.45 Da        Isoelectric Point: 8.9130

>T32026 WP_014386536.1 NZ_AP018748:c83368-82886 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Download         Length: 483 bp

>T32026 NZ_AP018748:c83368-82886 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10704.23 Da        Isoelectric Point: 4.5271

>AT32026 WP_004902250.1 NZ_AP018748:c83637-83359 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT32026 NZ_AP018748:c83637-83359 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBI1

Antitoxin

Source ID Structure
AlphaFold DB A0A071LPN3

References