Detailed information of TA system TA032026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 82886..83637
• Replicon: plasmid pKP3301
• Accession: NZ_AP018748
• Organism: Klebsiella pneumoniae strain KP33

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: H6U1U8
• Locus tag: D5079_RS26675
• Protein ID: WP_014386536.1
• Coordinates: 82886..83368 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: D5079_RS26680
• Protein ID: WP_004902250.1
• Coordinates: 83359..83637 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5079_RS26645	79285..79932	-	648	WP_014386537.1	EcsC family protein	-
D5079_RS26650	79959..80714	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
D5079_RS26655	80815..81207	-	393	WP_032442757.1	hypothetical protein	-
D5079_RS26660	81312..81851	-	540	WP_004902239.1	hypothetical protein	-
D5079_RS26675	82886..83368	-	483	WP_014386536.1	GNAT family N-acetyltransferase	Toxin
D5079_RS26680	83359..83637	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
D5079_RS26685	83756..83968	-	213	WP_004902255.1	hypothetical protein	-
D5079_RS26690	84076..84417	-	342	WP_004902257.1	hypothetical protein	-
D5079_RS26700	85247..85705	-	459	WP_014386535.1	hypothetical protein	-
D5079_RS26705	86358..86813	-	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
D5079_RS26710	86885..87250	+	366	WP_004200999.1	mercuric ion transporter MerT	-
D5079_RS26715	87266..87541	+	276	WP_000732275.1	mercury resistance system periplasmic binding protein MerP	-
D5079_RS26720	87569..87994	+	426	WP_000522996.1	organomercurial transporter MerC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / aac(6')-Ib-cr / blaOXA-1	-	1..296750	296750

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17662.45 Da
• Isoelectric Point: 8.9130

>T32026 WP_014386536.1 NZ_AP018748:c83368-82886 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T32026 NZ_AP018748:c83368-82886 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT32026 WP_004902250.1 NZ_AP018748:c83637-83359 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT32026 NZ_AP018748:c83637-83359 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• AlphaFold DB: A0A2A5MBI1

Antitoxin

• AlphaFold DB: A0A071LPN3