Detailed information of TA system TA032025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 6587..7497
• Replicon: plasmid pKP3301
• Accession: NZ_AP018748
• Organism: Klebsiella pneumoniae strain KP33

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: D5079_RS26260
• Protein ID: WP_004181896.1
• Coordinates: 7027..7497 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: D5079_RS26255
• Protein ID: WP_004181895.1
• Coordinates: 6587..7030 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5079_RS26235	1869..2576	+	708	WP_062826779.1	hypothetical protein	-
D5079_RS26240	2581..3612	+	1032	WP_170856552.1	CRISPR-associated protein Csf2	-
D5079_RS26245	4793..5215	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
D5079_RS26250	5215..6486	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
D5079_RS26255	6587..7030	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
D5079_RS26260	7027..7497	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
D5079_RS26265	7607..7867	-	261	WP_004026352.1	hypothetical protein	-
D5079_RS26275	8590..8748	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
D5079_RS29330	9351..10325	+	975	WP_043907013.1	hypothetical protein	-
D5079_RS26290	10523..10900	-	378	WP_004181901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / aac(6')-Ib-cr / blaOXA-1	-	1..296750	296750

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T32025 WP_004181896.1 NZ_AP018748:7027-7497 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T32025 NZ_AP018748:7027-7497 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT32025 WP_004181895.1 NZ_AP018748:6587-7030 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT32025 NZ_AP018748:6587-7030 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9