Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3649093..3649712 Replicon chromosome
Accession NZ_AP018747
Organism Klebsiella pneumoniae strain KP33

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag D5079_RS18450 Protein ID WP_002892050.1
Coordinates 3649494..3649712 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag D5079_RS18445 Protein ID WP_002892066.1
Coordinates 3649093..3649467 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D5079_RS18435 3644246..3645438 + 1193 Protein_3533 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
D5079_RS18440 3645461..3648607 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
D5079_RS18445 3649093..3649467 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
D5079_RS18450 3649494..3649712 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
D5079_RS18455 3649871..3650437 + 567 WP_002892030.1 maltose O-acetyltransferase -
D5079_RS29195 3650409..3650549 - 141 WP_004147370.1 hypothetical protein -
D5079_RS18460 3650570..3651040 + 471 WP_002892026.1 YlaC family protein -
D5079_RS18465 3651015..3652464 - 1450 Protein_3540 PLP-dependent aminotransferase family protein -
D5079_RS18470 3652565..3653263 + 699 WP_004178762.1 GNAT family N-acetyltransferase -
D5079_RS18475 3653260..3653400 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
D5079_RS18480 3653400..3653663 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T32020 WP_002892050.1 NZ_AP018747:3649494-3649712 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T32020 NZ_AP018747:3649494-3649712 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT32020 WP_002892066.1 NZ_AP018747:3649093-3649467 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT32020 NZ_AP018747:3649093-3649467 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References