Detailed information of TA system TA032010

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 480011..480668
• Replicon: chromosome
• Accession: NZ_AP018747
• Organism: Klebsiella pneumoniae strain KP33

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: D5079_RS02525
• Protein ID: WP_004181233.1
• Coordinates: 480258..480668 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: D5079_RS02520
• Protein ID: WP_002916312.1
• Coordinates: 480011..480277 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5079_RS02495	475167..476600	-	1434	WP_004181234.1	6-phospho-beta-glucosidase	-
D5079_RS02500	476719..477447	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
D5079_RS02505	477497..477808	+	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
D5079_RS02510	477972..478631	+	660	WP_002916317.1	hemolysin III family protein	-
D5079_RS02515	478782..479765	-	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
D5079_RS02520	480011..480277	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
D5079_RS02525	480258..480668	+	411	WP_004181233.1	protein YgfX	Toxin
D5079_RS02530	480675..481196	-	522	WP_004144730.1	flavodoxin FldB	-
D5079_RS02535	481297..482193	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
D5079_RS02540	482216..482929	+	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
D5079_RS02545	482935..484668	+	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16121.92 Da
• Isoelectric Point: 11.1565

>T32010 WP_004181233.1 NZ_AP018747:480258-480668 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAEEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T32010 NZ_AP018747:480258-480668 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGAAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT32010 WP_002916312.1 NZ_AP018747:480011-480277 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT32010 NZ_AP018747:480011-480277 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41