Detailed information of TA system TA032004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 120739..121260
• Replicon: plasmid pR09L01
• Accession: NZ_AP018735
• Organism: Rhodococcus erythropolis strain JCM 2895

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: T5HL71
• Locus tag: RE2895_RS30930
• Protein ID: WP_021334703.1
• Coordinates: 120955..121260 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: RE2895_RS30925
• Protein ID: WP_030538123.1
• Coordinates: 120739..120954 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RE2895_RS30900 (RE2895_61840)	116610..116819	+	210	WP_166503019.1	hypothetical protein	-
RE2895_RS30905	116740..117042	-	303	Protein_123	transposase	-
RE2895_RS30910 (RE2895_61850)	117081..118394	-	1314	WP_030538128.1	IS256 family transposase	-
RE2895_RS30915 (RE2895_61860)	118532..119314	-	783	WP_021334706.1	DUF4145 domain-containing protein	-
RE2895_RS30920 (RE2895_61870)	119656..120375	+	720	WP_021334705.1	hypothetical protein	-
RE2895_RS30925 (RE2895_61880)	120739..120954	+	216	WP_030538123.1	hypothetical protein	Antitoxin
RE2895_RS30930 (RE2895_61890)	120955..121260	+	306	WP_021334703.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RE2895_RS30935 (RE2895_61900)	121289..123256	+	1968	WP_021334702.1	hypothetical protein	-
RE2895_RS30940 (RE2895_61910)	123256..124053	+	798	WP_021334701.1	hypothetical protein	-
RE2895_RS30945 (RE2895_61920)	124179..124526	-	348	WP_021334700.1	PDDEXK nuclease domain-containing protein	-
RE2895_RS30950 (RE2895_61940)	124837..125895	+	1059	WP_165639875.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..227989	227989

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 10515.97 Da
• Isoelectric Point: 4.7318

>T32004 WP_021334703.1 NZ_AP018735:120955-121260 [Rhodococcus erythropolis]
VRPIHDAQLGAGVSSVLVLTRELVRPHLGTVTVAPIRAEGRGLSTELSLGEGNGVEDGSVVDCDGLTTIQVSALGVNRGF
LLPAQEVLLTRALRSAFDLES

Nucleotide

• Length: 306 bp

>T32004 NZ_AP018735:120955-121260 [Rhodococcus erythropolis]
GTGCGCCCGATCCACGATGCGCAGCTCGGCGCGGGCGTTTCTTCGGTGTTGGTTCTGACGAGAGAGTTGGTTCGGCCTCA
TCTGGGGACGGTTACTGTTGCGCCGATTCGTGCGGAGGGGCGGGGGTTGTCGACGGAGCTGAGTTTGGGTGAAGGCAACG
GCGTCGAGGACGGATCTGTTGTCGATTGTGATGGGTTGACGACGATCCAGGTGTCGGCTCTCGGTGTGAACCGGGGGTTT
CTGCTGCCGGCGCAGGAAGTGTTGTTGACAAGGGCATTGCGATCAGCGTTCGACCTCGAATCATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7525.58 Da
• Isoelectric Point: 10.4526

>AT32004 WP_030538123.1 NZ_AP018735:120739-120954 [Rhodococcus erythropolis]
MSTQIAVRLPEGIVAFLDGEVSGGRAKSRAQVVLRALEREQRRQLAVRDVEILSDGAGGDVNIAAAHGKKS

Nucleotide

• Length: 216 bp

>AT32004 NZ_AP018735:120739-120954 [Rhodococcus erythropolis]
ATGAGTACGCAGATCGCGGTACGGCTGCCCGAGGGCATTGTGGCGTTTCTCGACGGTGAGGTCAGCGGCGGGCGCGCGAA
AAGCCGTGCCCAAGTCGTGCTTCGAGCATTGGAGCGTGAGCAGCGTCGGCAGTTGGCGGTCCGTGACGTGGAGATCTTGT
CTGACGGCGCAGGCGGCGATGTGAATATCGCTGCCGCGCACGGGAAGAAGAGCTGA

Structures

Toxin

• AlphaFold DB: T5HL71