Detailed information of TA system TA031989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 147269..147840
• Replicon: chromosome
• Accession: NZ_AP018722
• Organism: Thiomicrorhabdus aquaedulcis strain HaS4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EP181_RS00590
• Protein ID: WP_127469936.1
• Coordinates: 147598..147840 (-)
• Length: 81 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EP181_RS00585
• Protein ID: WP_127469935.1
• Coordinates: 147269..147601 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP181_RS00560	142609..143667	-	1059	WP_172959649.1	phospholipase A	-
EP181_RS00565	143667..144353	-	687	WP_127469931.1	hypothetical protein	-
EP181_RS00570	144368..145189	-	822	WP_127469932.1	exodeoxyribonuclease III	-
EP181_RS00575	145401..146258	+	858	WP_127469933.1	formyltetrahydrofolate deformylase	-
EP181_RS00580	146359..147090	+	732	WP_127469934.1	zeta toxin family protein	-
EP181_RS00585	147269..147601	-	333	WP_127469935.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EP181_RS00590	147598..147840	-	243	WP_127469936.1	type II toxin-antitoxin system HicA family toxin	Toxin
EP181_RS00595	148076..149146	+	1071	WP_127469937.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
EP181_RS00600	149283..151661	-	2379	WP_127469938.1	tRNA(Met) cytidine acetyltransferase TmcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-74)

Antitoxin

• Uncharacterised protein family HicB (55-103)

Sequences

Toxin

Protein

• Length: 81 a.a.
• Molecular weight: 9257.75 Da
• Isoelectric Point: 9.6580

>T31989 WP_127469936.1 NZ_AP018722:c147840-147598 [Thiomicrorhabdus aquaedulcis]
MSKKDKLLQKFLATKRNFLWSDVVGVLTALGFEQIEAEGSRVDFVKGDFIINLHKPHPSKEVKEYALKQLKEQLNQWGEL

Nucleotide

• Length: 243 bp

>T31989 NZ_AP018722:c147840-147598 [Thiomicrorhabdus aquaedulcis]
ATGAGCAAAAAAGATAAGCTATTGCAAAAGTTTTTAGCTACTAAACGCAACTTTTTATGGTCAGACGTGGTGGGAGTGCT
CACGGCTTTGGGTTTCGAGCAAATAGAAGCGGAAGGCTCTAGAGTTGATTTTGTAAAAGGTGATTTCATCATTAACCTGC
ATAAGCCGCACCCGTCTAAAGAGGTTAAGGAGTATGCTCTTAAGCAACTTAAAGAACAGCTAAATCAATGGGGTGAACTA
TGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12313.06 Da
• Isoelectric Point: 5.6394

>AT31989 WP_127469935.1 NZ_AP018722:c147601-147269 [Thiomicrorhabdus aquaedulcis]
MSEFMEYKGYLGSVEYSAQDHCLYGKLAYIRALVNYEAQTVEELEREFCSAVDHYLAVCEKLGKTPQTPLKGTFNIRISP
ELHRAAVMAAGNKSLNAFVAEAIEFRLAQG

Nucleotide

• Length: 333 bp

>AT31989 NZ_AP018722:c147601-147269 [Thiomicrorhabdus aquaedulcis]
ATGAGCGAATTCATGGAATATAAGGGTTATTTAGGGTCGGTTGAATACAGCGCCCAAGACCACTGCTTGTACGGAAAATT
GGCGTACATTCGCGCGTTGGTAAATTACGAGGCGCAAACGGTTGAAGAGTTGGAACGTGAGTTTTGTTCTGCCGTTGACC
ATTATTTGGCCGTCTGCGAAAAGCTTGGCAAGACGCCTCAAACCCCACTAAAGGGTACGTTTAATATTAGAATTAGCCCA
GAGCTGCATAGGGCGGCGGTGATGGCGGCGGGTAATAAAAGCTTAAACGCGTTTGTGGCCGAAGCGATTGAGTTCCGCCT
AGCGCAGGGTTAA