Detailed information of TA system TA031970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 944..1415
• Replicon: plasmid 2
• Accession: NZ_AP018683
• Organism: Vibrio casei strain DSM 22364

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: VCASEI_RS19235
• Protein ID: WP_089111020.1
• Coordinates: 1152..1415 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: VCASEI_RS19230
• Protein ID: WP_089111021.1
• Coordinates: 944..1168 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCASEI_RS19230	944..1168	+	225	WP_089111021.1	TraY domain-containing protein	Antitoxin
VCASEI_RS19235	1152..1415	+	264	WP_089111020.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VCASEI_RS19240	1520..1804	-	285	WP_089111019.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCASEI_RS19245	1794..2033	-	240	WP_089111018.1	CopG family ribbon-helix-helix protein	-
VCASEI_RS19250	2190..2525	-	336	Protein_4	transposase	-
VCASEI_RS19265	3085..4263	+	1179	WP_001572339.1	FMN-dependent L-lactate dehydrogenase LldD	-
VCASEI_RS19275	5352..6227	-	876	Protein_6	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..16253	16253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• Protein of unknown function DUF6290 (3-70)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10139.76 Da
• Isoelectric Point: 9.8948

>T31970 WP_089111020.1 NZ_AP018683:1152-1415 [Vibrio casei]
MAWTIEYSLKAVKELKKLDKKAASTIIDYLDGIAQLDDPRSKGKSLRGDKRQFWRYRVGNYRVLCSIEDDELIILATHLG
HRKNVYL

Nucleotide

• Length: 264 bp

>T31970 NZ_AP018683:1152-1415 [Vibrio casei]
TTGGCTTGGACGATTGAGTATTCTTTAAAAGCAGTAAAAGAATTAAAGAAGCTCGATAAGAAAGCAGCATCTACGATTAT
TGATTATTTGGATGGTATTGCACAATTAGATGACCCTCGTTCAAAAGGTAAATCTTTGAGAGGAGATAAGCGTCAATTTT
GGCGGTATCGAGTGGGTAACTATAGAGTATTATGCAGTATTGAAGATGATGAACTTATTATTCTTGCTACACACCTAGGG
CATAGGAAAAATGTGTATTTATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8587.59 Da
• Isoelectric Point: 4.6510

>AT31970 WP_089111021.1 NZ_AP018683:944-1168 [Vibrio casei]
MLAIRLPEDIENRLSHLAQETGRTKTYYAREAILKHLSNLEDYYLAENVSQKVKNGEMETYSADEVRKELGLDD

Nucleotide

• Length: 225 bp

>AT31970 NZ_AP018683:944-1168 [Vibrio casei]
ATGTTAGCTATTCGACTACCTGAAGATATTGAAAATAGATTATCTCATTTAGCCCAAGAGACAGGCAGAACGAAAACTTA
CTATGCGAGAGAGGCAATACTTAAGCATCTTTCTAATCTTGAAGATTACTACCTGGCGGAAAATGTTTCTCAAAAAGTGA
AAAATGGAGAAATGGAAACTTATTCAGCTGATGAAGTAAGGAAAGAACTTGGCTTGGACGATTGA