Detailed information of TA system TA031947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 4434..5014
• Replicon: plasmid pGSU10-3-4
• Accession: NZ_AP018675
• Organism: Klebsiella pneumoniae strain GSU10-3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: D6O76_RS30140
• Protein ID: WP_071177730.1
• Coordinates: 4700..5014 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: D6O76_RS30135
• Protein ID: WP_000093040.1
• Coordinates: 4434..4712 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6O76_RS30105	494..643	-	150	WP_032440454.1	colicin release lysis protein	-
D6O76_RS30110	728..985	-	258	WP_032440455.1	cloacin	-
D6O76_RS30115	995..2680	-	1686	WP_032440457.1	cloacin	-
D6O76_RS30120	3007..3252	+	246	WP_032440458.1	hypothetical protein	-
D6O76_RS30125	3526..3897	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
D6O76_RS30130	3894..4100	+	207	Protein_5	TonB family protein	-
D6O76_RS30135	4434..4712	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
D6O76_RS30140	4700..5014	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D6O76_RS30145	5178..5606	+	429	WP_001140599.1	hypothetical protein	-
D6O76_RS30150	5632..5811	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
D6O76_RS30155	5838..6368	-	531	WP_071177729.1	hypothetical protein	-
D6O76_RS30160	6375..7106	-	732	WP_071177728.1	replication-relaxation family protein	-
D6O76_RS30165	7106..9070	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T31947 WP_071177730.1 NZ_AP018675:4700-5014 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T31947 NZ_AP018675:4700-5014 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT31947 WP_000093040.1 NZ_AP018675:4434-4712 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT31947 NZ_AP018675:4434-4712 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1