Detailed information of TA system TA031946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 62304..62829
• Replicon: plasmid pGSU10-3-2
• Accession: NZ_AP018673
• Organism: Klebsiella pneumoniae strain GSU10-3

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: D6O76_RS29100
• Protein ID: WP_004197633.1
• Coordinates: 62524..62829 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: D6O76_RS29095
• Protein ID: WP_004197642.1
• Coordinates: 62304..62522 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6O76_RS29040	57410..58405	+	996	WP_019725651.1	hypothetical protein	-
D6O76_RS29045	58478..58801	+	324	WP_019725650.1	hypothetical protein	-
D6O76_RS31000	59235..59429	+	195	WP_019725033.1	hypothetical protein	-
D6O76_RS29055	59473..59703	-	231	WP_019725034.1	hypothetical protein	-
D6O76_RS29060	59717..59920	-	204	WP_019725040.1	hemolysin expression modulator Hha	-
D6O76_RS29065	59954..60322	-	369	WP_015065502.1	hypothetical protein	-
D6O76_RS29070	60366..60860	-	495	WP_009310053.1	hypothetical protein	-
D6O76_RS29075	60891..61463	-	573	WP_015065504.1	hypothetical protein	-
D6O76_RS29080	61460..61708	-	249	WP_019725036.1	hypothetical protein	-
D6O76_RS29095	62304..62522	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
D6O76_RS29100	62524..62829	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
D6O76_RS29105	62998..63399	+	402	WP_072199347.1	hypothetical protein	-
D6O76_RS29110	63426..63749	+	324	WP_004197641.1	hypothetical protein	-
D6O76_RS29115	63746..64762	+	1017	WP_004197639.1	hypothetical protein	-
D6O76_RS29120	64960..65754	+	795	WP_004197635.1	site-specific integrase	-
D6O76_RS29125	66202..66381	-	180	WP_006788217.1	hypothetical protein	-
D6O76_RS29130	66501..67127	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa	-	1..134879	134879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T31946 WP_004197633.1 NZ_AP018673:62524-62829 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T31946 NZ_AP018673:62524-62829 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT31946 WP_004197642.1 NZ_AP018673:62304-62522 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT31946 NZ_AP018673:62304-62522 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5