Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 32444..33180 Replicon plasmid pGSU10-3-2
Accession NZ_AP018673
Organism Klebsiella pneumoniae strain GSU10-3

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag D6O76_RS28880 Protein ID WP_003026803.1
Coordinates 32698..33180 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag D6O76_RS28875 Protein ID WP_003026799.1
Coordinates 32444..32710 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6O76_RS28860 27737..28942 - 1206 WP_004206572.1 chromate efflux transporter -
D6O76_RS28865 29118..29696 - 579 WP_040229494.1 recombinase family protein -
D6O76_RS28870 29855..32147 + 2293 Protein_35 Tn3 family transposase -
D6O76_RS28875 32444..32710 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
D6O76_RS28880 32698..33180 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
D6O76_RS28885 33296..33466 + 171 Protein_38 ISNCY family transposase -
D6O76_RS28890 33500..35251 - 1752 Protein_39 Tn3-like element TnAs1 family transposase -
D6O76_RS28895 35240..35647 + 408 WP_001214976.1 cysteine hydrolase -
D6O76_RS28900 35785..36669 + 885 WP_000058717.1 EamA family transporter -
D6O76_RS28905 36701..37900 - 1200 WP_000804064.1 tetracycline efflux MFS transporter Tet(A) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa - 1..134879 134879
- inside IScluster/Tn tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa - 29118..55962 26844


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T31945 WP_003026803.1 NZ_AP018673:32698-33180 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T31945 NZ_AP018673:32698-33180 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT31945 WP_003026799.1 NZ_AP018673:32444-32710 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT31945 NZ_AP018673:32444-32710 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References