Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5400802..5401427 Replicon chromosome
Accession NZ_AP018671
Organism Klebsiella pneumoniae strain GSU10-3

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag D6O76_RS27290 Protein ID WP_002882817.1
Coordinates 5400802..5401185 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag D6O76_RS27295 Protein ID WP_004150355.1
Coordinates 5401185..5401427 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6O76_RS27275 5398168..5399070 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
D6O76_RS27280 5399067..5399702 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
D6O76_RS27285 5399699..5400628 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
D6O76_RS27290 5400802..5401185 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
D6O76_RS27295 5401185..5401427 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
D6O76_RS27300 5401632..5402549 + 918 WP_002882812.1 alpha/beta hydrolase -
D6O76_RS27305 5402563..5403504 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
D6O76_RS27310 5403549..5403986 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
D6O76_RS27315 5403983..5404843 - 861 WP_002882807.1 virulence factor BrkB family protein -
D6O76_RS27320 5404837..5405436 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T31943 WP_002882817.1 NZ_AP018671:c5401185-5400802 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T31943 NZ_AP018671:c5401185-5400802 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT31943 WP_004150355.1 NZ_AP018671:c5401427-5401185 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT31943 NZ_AP018671:c5401427-5401185 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References