Detailed information of TA system TA031925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 160636..161167
• Replicon: plasmid pSAMIE_3
• Accession: NZ_AP018666
• Organism: Sphingobium amiense strain DSM 16289

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T0GNF5
• Locus tag: SAMIE_RS22245
• Protein ID: WP_004212690.1
• Coordinates: 160636..160917 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: T0G968
• Locus tag: SAMIE_RS22250
• Protein ID: WP_004212689.1
• Coordinates: 160904..161167 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAMIE_RS22225	155983..158250	+	2268	WP_025549136.1	nitric-oxide reductase large subunit	-
SAMIE_RS22230	158960..159418	-	459	WP_004212694.1	TonB-dependent receptor	-
SAMIE_RS22235	159590..159910	-	321	Protein_170	ATP-binding protein	-
SAMIE_RS22240	159911..160234	-	324	Protein_171	IS21 family transposase	-
SAMIE_RS23590	160232..160315	-	84	Protein_172	magnesium transporter	-
SAMIE_RS22245	160636..160917	-	282	WP_004212690.1	type II toxin-antitoxin system YafQ family toxin	Toxin
SAMIE_RS22250	160904..161167	-	264	WP_004212689.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SAMIE_RS22255	161551..161988	-	438	WP_007683347.1	metal-binding protein	-
SAMIE_RS22260	162068..162604	-	537	Protein_176	MFS transporter	-
SAMIE_RS22265	162726..165635	+	2910	WP_126516964.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..212121	212121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10993.49 Da
• Isoelectric Point: 7.4752

>T31925 WP_004212690.1 NZ_AP018666:c160917-160636 [Sphingobium amiense]
MRTIERFGRFKRDYKREKKGQHAKTLDADLIPIIEALASDEPLEPRHRDHALTGDWRDHRDCHIKPDLVLIYRKPDDDTL
QLVRLGSHAELGW

Nucleotide

• Length: 282 bp

>T31925 NZ_AP018666:c160917-160636 [Sphingobium amiense]
ATGCGGACGATTGAACGCTTCGGGCGGTTCAAGCGCGACTACAAGCGGGAGAAGAAGGGGCAGCACGCCAAGACGCTGGA
CGCTGACCTGATCCCGATCATCGAGGCGCTGGCATCCGACGAGCCGCTTGAACCGCGCCACCGCGACCATGCGCTGACCG
GCGACTGGCGCGACCATCGCGATTGCCACATAAAGCCCGACCTCGTGCTGATTTACCGCAAGCCCGATGACGACACCTTG
CAGCTTGTGCGCTTGGGATCGCACGCGGAATTGGGTTGGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9284.75 Da
• Isoelectric Point: 4.5881

>AT31925 WP_004212689.1 NZ_AP018666:c161167-160904 [Sphingobium amiense]
MAANALVQTRIDGAVKEEAATVLAAMGLTVSDAVRLMLTRVARDKALPFEPLVPNAETIEAMKEAREGKGKRFATVVDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT31925 NZ_AP018666:c161167-160904 [Sphingobium amiense]
ATGGCCGCAAACGCTCTTGTGCAGACCCGGATCGACGGAGCGGTGAAGGAGGAAGCCGCGACCGTGCTGGCTGCTATGGG
GCTGACCGTCTCCGATGCCGTGCGCCTGATGCTGACCCGCGTTGCCCGCGACAAGGCCTTGCCGTTTGAACCGCTGGTGC
CCAATGCCGAGACGATCGAGGCCATGAAGGAAGCGCGCGAGGGAAAGGGCAAGCGGTTCGCCACGGTGGTCGACCTGATG
GCCGACCTCAATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: T0GNF5

Antitoxin

• AlphaFold DB: T0G968