Detailed information of TA system TA031924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 33738..34297
• Replicon: plasmid pSAMIE_2
• Accession: NZ_AP018665
• Organism: Sphingobium amiense strain DSM 16289

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: SAMIE_RS20440
• Protein ID: WP_066704026.1
• Coordinates: 33738..34031 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: SAMIE_RS20445
• Protein ID: WP_066704024.1
• Coordinates: 34019..34297 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAMIE_RS20410	29180..30775	+	1596	WP_162849111.1	FAD-dependent monooxygenase	-
SAMIE_RS20415	31123..31473	-	351	WP_123905555.1	hypothetical protein	-
SAMIE_RS20420	31451..31735	-	285	WP_145988141.1	TetR/AcrR family transcriptional regulator	-
SAMIE_RS20425	31748..32116	+	369	WP_066704032.1	CopG family transcriptional regulator	-
SAMIE_RS20430	32122..33102	+	981	WP_066704038.1	tyrosine-type recombinase/integrase	-
SAMIE_RS20435	33099..33719	-	621	WP_066704029.1	phosphoglycerate mutase family protein	-
SAMIE_RS20440	33738..34031	-	294	WP_066704026.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SAMIE_RS20445	34019..34297	-	279	WP_066704024.1	CopG family ribbon-helix-helix protein	Antitoxin
SAMIE_RS20450	34837..35478	+	642	WP_066704021.1	transcription elongation protein SprT	-
SAMIE_RS20455	35558..36193	+	636	WP_066704017.1	AAA family ATPase	-
SAMIE_RS20460	36219..36500	+	282	WP_066704014.1	hypothetical protein	-
SAMIE_RS20465	36557..37468	-	912	WP_066704011.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..212396	212396
	-	flank	IS/Tn	-	-	37776..39872	2096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10846.42 Da
• Isoelectric Point: 9.4809

>T31924 WP_066704026.1 NZ_AP018665:c34031-33738 [Sphingobium amiense]
VPRLIWTANALNNVDRLHRWLKPKDADAARRAVAAIRGGVKILATSPHIGRPAEDMDPEYREKLIDFGDSGYVVLYRLDG
EAVTLLAVRHQKEAGYP

Nucleotide

• Length: 294 bp

>T31924 NZ_AP018665:c34031-33738 [Sphingobium amiense]
GTGCCACGTCTGATCTGGACGGCGAACGCGCTGAATAATGTTGACCGGCTGCATCGCTGGTTGAAGCCCAAGGATGCGGA
CGCCGCGCGCCGCGCGGTCGCAGCGATCCGGGGAGGGGTCAAGATTCTGGCGACATCGCCGCACATCGGGCGGCCTGCGG
AAGATATGGACCCCGAATATCGGGAAAAGCTGATCGACTTTGGAGATAGCGGCTATGTCGTCCTCTATCGCCTCGATGGC
GAGGCGGTGACGCTGCTGGCGGTTCGCCACCAAAAGGAGGCCGGCTATCCGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10605.88 Da
• Isoelectric Point: 5.1991

>AT31924 WP_066704024.1 NZ_AP018665:c34297-34019 [Sphingobium amiense]
MAAATSIKLDDSLKGRVQHLADARRRSSHWIMREAITQYVDREEKREAFKQEAIQAWEEYQQTGLHVTLEEADAWLAKLE
AGEDAELPKCHV

Nucleotide

• Length: 279 bp

>AT31924 NZ_AP018665:c34297-34019 [Sphingobium amiense]
ATGGCGGCGGCAACATCTATCAAGCTGGACGATAGCCTGAAAGGGCGAGTGCAACACTTGGCCGACGCGCGCCGGCGCAG
CTCGCATTGGATCATGCGCGAGGCCATCACGCAGTATGTCGATCGCGAGGAAAAGCGCGAGGCGTTCAAGCAGGAAGCCA
TACAGGCATGGGAGGAATATCAGCAGACCGGCTTGCATGTGACGCTTGAGGAAGCCGACGCCTGGTTGGCGAAGCTGGAA
GCGGGCGAGGACGCGGAACTACCTAAGTGCCACGTCTGA