Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3563724..3564537 Replicon chromosome
Accession NZ_AP018664
Organism Sphingobium amiense strain DSM 16289

Toxin (Protein)


Gene name tacT Uniprot ID A0A494WFG0
Locus tag SAMIE_RS17255 Protein ID WP_015740123.1
Coordinates 3564028..3564537 (+) Length 170 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A4P6S0J1
Locus tag SAMIE_RS17250 Protein ID WP_066478980.1
Coordinates 3563724..3564023 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAMIE_RS17240 3561585..3563210 - 1626 Protein_3407 recombinase family protein -
SAMIE_RS17245 3563230..3563454 - 225 WP_066478981.1 hypothetical protein -
SAMIE_RS17250 3563724..3564023 + 300 WP_066478980.1 DUF1778 domain-containing protein Antitoxin
SAMIE_RS17255 3564028..3564537 + 510 WP_015740123.1 GNAT family N-acetyltransferase Toxin
SAMIE_RS17260 3564695..3566038 + 1344 WP_011578756.1 IS1380-like element ISMesp2 family transposase -
SAMIE_RS17265 3566435..3567328 - 894 WP_066704005.1 3'-5' exonuclease -
SAMIE_RS17270 3567315..3569345 - 2031 WP_066704003.1 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3564695..3566038 1343


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Vibrio phage ICP1, Orf50

(12-86)


Sequences

Toxin        


Download         Length: 170 a.a.        Molecular weight: 17926.66 Da        Isoelectric Point: 8.2975

>T31923 WP_015740123.1 NZ_AP018664:3564028-3564537 [Sphingobium amiense]
MLLSGPELLSAAHDVSQFSCGKPTLDHWLKTRALSNQQKGFTAVLVVHEAGRVVGYYGLAPTAVVPSVLPRSIRTGQPPN
PIPCMLLGQLATDIGSAGLGIGTGLVKHALQRCVQAASLIGGRALMVNAVDGEAAEFWQRRGFEPTRDDELVLFRSISDI
AASLREAGR

Download         Length: 510 bp

>T31923 NZ_AP018664:3564028-3564537 [Sphingobium amiense]
TTGCTGCTTTCAGGACCCGAGCTGCTTTCCGCCGCCCACGATGTTTCCCAGTTTTCCTGTGGCAAGCCGACGCTCGACCA
CTGGCTGAAGACCCGCGCGCTCTCCAATCAGCAGAAGGGCTTTACCGCCGTTCTCGTCGTGCATGAGGCAGGGCGTGTGG
TGGGATATTACGGCCTTGCACCAACAGCCGTTGTGCCATCGGTGCTACCGCGCTCCATCCGGACGGGACAGCCGCCTAAT
CCAATTCCCTGCATGCTGCTTGGTCAGCTCGCGACCGATATCGGCTCGGCCGGGTTGGGTATCGGGACTGGCCTTGTGAA
GCACGCCCTCCAACGGTGCGTTCAGGCCGCATCGCTGATCGGTGGGCGGGCATTGATGGTCAACGCCGTCGACGGGGAGG
CTGCCGAATTCTGGCAGCGCCGAGGATTTGAGCCTACCAGAGATGATGAACTGGTCCTGTTTCGCTCGATCAGCGATATT
GCTGCGTCGCTCCGTGAGGCTGGAAGGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11060.78 Da        Isoelectric Point: 5.1972

>AT31923 WP_066478980.1 NZ_AP018664:3563724-3564023 [Sphingobium amiense]
MANTAERKEYPISMRLPEADVAMIDRAASLRGRSRTDFVRDAAVRAAEEVVMEQGLIRMSPEGFAQFMDVLSRPVAPVPE
MVEVLKRPAPWEPGYAAKQ

Download         Length: 300 bp

>AT31923 NZ_AP018664:3563724-3564023 [Sphingobium amiense]
ATGGCCAACACTGCCGAACGCAAGGAATATCCGATCTCAATGCGTCTGCCCGAGGCCGATGTTGCCATGATCGATCGTGC
TGCCAGCCTGCGCGGCCGCTCGCGCACCGATTTCGTGCGCGACGCCGCTGTGCGCGCGGCCGAGGAGGTCGTCATGGAGC
AGGGCCTGATCCGGATGAGCCCGGAAGGCTTTGCCCAGTTCATGGATGTGCTGTCGCGTCCGGTTGCTCCTGTTCCGGAG
ATGGTTGAAGTGCTTAAGCGGCCTGCGCCGTGGGAGCCCGGCTACGCAGCGAAACAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A494WFG0

Antitoxin

Source ID Structure
AlphaFold DB A0A4P6S0J1

References