Detailed information of TA system TA031921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1084550..1085127
• Replicon: chromosome
• Accession: NZ_AP018664
• Organism: Sphingobium amiense strain DSM 16289

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A494VYC8
• Locus tag: SAMIE_RS05090
• Protein ID: WP_066699765.1
• Coordinates: 1084792..1085127 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: SAMIE_RS05085
• Protein ID: WP_066699767.1
• Coordinates: 1084550..1084792 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAMIE_RS05060	1080441..1081343	+	903	WP_030538914.1	DUF1738 domain-containing protein	-
SAMIE_RS05065	1081340..1081654	+	315	WP_126516754.1	antirestriction protein ArdC	-
SAMIE_RS05070	1081651..1082052	+	402	WP_066699794.1	DUF2958 domain-containing protein	-
SAMIE_RS05075	1082169..1084043	+	1875	WP_066699793.1	ParB N-terminal domain-containing protein	-
SAMIE_RS05080	1084225..1084425	+	201	WP_126516755.1	hypothetical protein	-
SAMIE_RS05085	1084550..1084792	+	243	WP_066699767.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SAMIE_RS05090	1084792..1085127	+	336	WP_066699765.1	endoribonuclease MazF	Toxin
SAMIE_RS05095	1085967..1086287	+	321	WP_066699763.1	DUF736 domain-containing protein	-
SAMIE_RS05100	1086464..1086721	+	258	WP_066699761.1	helix-turn-helix domain-containing protein	-
SAMIE_RS05105	1086718..1087290	+	573	WP_066699759.1	S26 family signal peptidase	-
SAMIE_RS05110	1087278..1087928	+	651	WP_066699757.1	lytic transglycosylase domain-containing protein	-
SAMIE_RS05115	1088046..1088711	+	666	WP_066699752.1	pentapeptide repeat-containing protein	-
SAMIE_RS23290	1088822..1089160	-	339	WP_066699751.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1024373..1109115	84742

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12025.02 Da
• Isoelectric Point: 9.5826

>T31921 WP_066699765.1 NZ_AP018664:1084792-1085127 [Sphingobium amiense]
MASRYIPDTGDIVWLQFDPQAGHEQAGHRPALVLSPAAYNKLRGMMICCPMTSQIKGYPFEVTVSQAPPSIVLSDQIKSL
DWKARGAKKKGSVSTAVIDEVRAKISALLGL

Nucleotide

• Length: 336 bp

>T31921 NZ_AP018664:1084792-1085127 [Sphingobium amiense]
ATGGCCAGTCGCTATATTCCGGACACTGGCGATATTGTGTGGCTGCAATTCGATCCCCAGGCCGGACATGAGCAAGCGGG
GCATCGTCCTGCGCTCGTCCTCTCACCTGCGGCCTACAACAAGCTGCGGGGCATGATGATTTGCTGCCCGATGACGAGCC
AGATCAAGGGCTATCCTTTCGAAGTGACCGTGTCCCAGGCACCGCCCAGCATCGTGCTGTCCGATCAGATCAAGAGCCTC
GATTGGAAGGCGCGGGGAGCGAAGAAAAAGGGCTCCGTATCAACGGCCGTGATCGATGAGGTGCGCGCCAAGATAAGCGC
ACTTCTGGGTCTTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8654.10 Da
• Isoelectric Point: 4.6653

>AT31921 WP_066699767.1 NZ_AP018664:1084550-1084792 [Sphingobium amiense]
MGIQVKKWGNSAAIRIPSAIMAAAALNVGQEVEVRQEGSRLIIEPVLKPVYTLDALLADMKPETFHEIVDFGPPVGNEVL

Nucleotide

• Length: 243 bp

>AT31921 NZ_AP018664:1084550-1084792 [Sphingobium amiense]
ATGGGTATTCAGGTAAAGAAGTGGGGCAATAGCGCTGCAATCCGGATTCCCTCCGCCATCATGGCGGCTGCCGCGCTGAA
TGTCGGTCAGGAGGTCGAAGTTCGCCAGGAAGGTAGCCGGCTCATCATCGAGCCCGTCCTGAAGCCTGTCTACACGCTCG
ACGCGTTGCTTGCGGACATGAAGCCGGAGACGTTCCACGAGATTGTCGATTTTGGTCCCCCGGTCGGCAATGAAGTGCTT
TGA

Structures

Toxin

• AlphaFold DB: A0A494VYC8